柴胡及其常见伪品的DNA指纹鉴定

Identification of Bupleurum chinese and Common Adulterants by DNA Fingerprint

目的:建立柴胡及其伪品DNA指纹鉴别方法。方法:采用改良的CTAB法提取柴胡及常见伪品的干根基因组DNA,设计鉴别正品柴胡及其伪品的特异性引物,利用PCR扩增技术对正品柴胡及其伪品进行DNA指纹鉴定。结果:改良的CTAB法提取出大小为23 kb的基因组DNA条带,纯度在(1.71±0.11),且经特异引物PCR扩增,只有正品柴胡能扩增出173bp大小的片段,而伪品未能扩增出目的片段。结论:柴胡DNA具有特异性指纹特征,可作为市售柴胡的真伪鉴定依据。

Objective： To establish DNA fingerprint method for the identification of Bupleurum chinese and adulterants.Method： The improved CTAB method was used to extract genomic DNA from B.chinese and common adulterants,designning the specific primer that identifies the B.chinese and common adulterants,and using DNA fingerprint Technology to identify the B.chinese and common adulterants by PCR.Result： The 23 kb DNA was separated from the B.chinese and common adulterantsthe by the improved CTAB,which purity was（1.71 ± 0.11）,the diagnostic results of PCR with specific primer showed that a 173 bp fragment was only amplified from the B.chinese.Conclusion： B.chinese DNA fingerprint has specific characteristics,it can be used for the crude drugs in the current market.