艾片口服给药后在小鼠体内的组织分布

Tissue Distribution of Borneol in Mice after Oral Administration

目的:建立龙脑在小鼠组织中GC-FID含量测定方法,考察艾片口服给药后在小鼠体内的组织分布情况。方法:小鼠按30.0 mg.kg-1灌胃给予艾片,分别于给药后1,3,5,10,20,30,60,90,120 min脱颈椎处死小鼠,迅速分离出小鼠全脑、心、肝、脾、肺、肾,匀浆后以十八烷为内标,乙酸乙酯萃取,采用GC测定各组织中龙脑质量浓度。结果:建立的含量测定方法中萃取回收率、日内和日间精密度、稳定性均符合生物样品的分析要求。艾片口服给药后分布于各主要脏器,组织中龙脑含量顺序为肝>肾>心>脑>脾>肺,龙脑在肝、肺、脾中达峰时间明显早于心、脑、肾。结论:建立的GC-FID适用于组织中龙脑的含量测定,艾片在不同组织内分布存在差异,在肝、肾、心、脑等血流丰富的组织中含量较高。

Objective： To develop a GC-FID method for determination of borneol concentration in mice tissues and investigate its tissues distribution after oral administration.Method： Wholebrain,heart,liver,spleen,lungs and kidneys were quickly isolated by executing mice at 1,3,5,10,20,30,60,90,120 min after oral administration of borneol with a dosage of 30.0 mg.kg-1.These tissues were extracted with ethyl acetate and detected by GC-FID,octadecane was used as an internal standard.Result： Calibration curve was good linear,extraction recoveries,RSD of within-day and between-day with low,medium and high concentration were satisfied for analysis requirements of biological samples.Borneol distributed in all major organs after administration with the order of liver kidney heart brain spleen lung,Tmax in liver,lung and spleen was earlier than in heart,brain and kidney.Conclusion： Established GC-FID could be applied to determination of borneol in tissues.There were differences of borneol distribution in each tissues,it mainly distributed in abundant bloodsupply tissues such as liver,kidneys,heart and brain.