溃结灵含药血清对Caco-2细胞损伤模型细胞增殖的影响

Effect of Kuijieling Decoction Contained Serum on Cell Proliferation in Caco-2 Cell Injury Model

目的:观察溃结灵含药血清对Caco-2细胞损伤模型细胞增殖的影响。方法:应用三硝基苯磺酸灌肠法复制溃疡性结肠炎大鼠模型,3 d后采集血液,分离血清,作为损伤剂作用于Caco-2细胞一段时间,模拟细胞炎性损伤模型,MTT法观察不同浓度溃结灵含药血清对Caco-2细胞损伤模型细胞增殖的影响。结果:与模型血清组比较,10%,5%,2.5%,1.25%,0.625%溃结灵含药血清作用Caco-2细胞损伤模型24 h后,有促进其增殖的作用,尤以10%溃结灵含药血清效果最佳(P<0.05)。结论:溃结灵含药血清可促进Caco-2细胞炎性损伤后细胞增殖。

Objective： To investigate the effect of Kuijieling decoction contained serum on cell proliferation in Caco-2 cell injury model.Method： Rectal instillation of trinitrobenzenesulfonic acid（TNBS） was used to establish the rats model of ulcerative colitis,3 days later blood simples were collected and serum was separated,which was used to intervene Caco-2 cells for a period of time to simulate cell inflammatory injury model,the effect of different concentrations of Kuijieling decoction contained serum oncell proliferation was observed by the method of MTT.Result： Compared with the model serum group,10%,5%,2.5%,1.25%,0.625% of Kuijieling decoction contained serum promoted proliferation,especially 10% of Kuijieling decoction contained serum was best（P 0.05）.Conclusion： Kuijieling decoction contained serum could promote the proliferation of Caco-2 cells after injury.