扇贝贝壳水提物对蛋白酶和成纤维细胞的体外作用研究

Reseach in vitro on Protease and TIG-101 Cells of Scallop Shells Bioactive Components

目的:体外研究扇贝贝壳水提物的皮肤保护作用。方法:提取扇贝贝壳的水溶性有机成分,体外考察对皮肤保护相关的蛋白酶活性以及成纤维细胞TIG-101增殖(MTT法)和胶原蛋白合成(染色法)的影响,评价扇贝贝壳所含生物活性物质对皮肤的保护作用。结果:扇贝贝壳水溶性有机成分可增强糜蛋白酶活性,抑制弹性蛋白酶和胰蛋白酶活性,并可促进皮肤上皮细胞增殖(P<0.05)和胶原蛋白合成增加(P<0.05)。结论:扇贝贝壳水溶性有机成分具有保护皮肤组织的生物活性。

Objective： The skin protection of scallop shells extract in vitro was studied.Method： There were some bioactive components in scallop shells.The extract of Patinopecten yessoensis scallop shell was used to evaluate the skin protection by observation of skin fibroblast cells TIG-101（MTT assay） and collagen synthesis （direct red 80%）.Result： The result indicated that the bioactive components of scallop shells could enhance the bioactivity of chymotrypsin,but had a strong inhibitory effect on the bioactivity of elastase and trypsin;enhance cell proliferation of the skin fibroblast（P 0.05） and collagen synthesis（P 0.05）.Conclusion： These results indicate that the components of the scallop shell contain the biological active substances that protect the skin tissue.