小柴胡汤不同萃取部位抗抑郁作用筛选

Screening of Antidepressant Effects of Different Fractions from Xiaochaihu Decoction

目的:对小柴胡汤的不同萃取部位进行抗抑郁活性筛选。方法:雄性昆明种小鼠随机分为10组,空白对照组、阳性对照盐酸氟西汀组(20 mg.kg-1)、小柴胡汤的石油醚萃取物(部位Ⅰ)、乙酸乙酯萃取物(部位Ⅱ)、水饱和正丁醇萃取物(部位Ⅲ)、水层残留物(部位Ⅳ),其中部位Ⅰ～Ⅳ各自分为9.0,18.0 g.kg-12个剂量组。连续ig 7 d。末次给药1 h后,分别采用小鼠悬尾和小鼠强迫游泳2种行为绝望试验,比较其抗抑郁药效。结果:部位Ⅱ、部位Ⅲ可以明显缩短小鼠强迫游泳不动时间、悬尾不动时间(P<0.05或P<0.01)。结论:结果初步确定乙酸乙酯萃取物和正丁醇萃取物为小柴胡汤抗抑郁作用的有效部位。

Objective： To screen the antidepressant effects of different fractions from Xiao Chaihu decoction.Method： Male Kunming mice were randomly divided into 10 groups： control group,fluoxetine and the petroleum ether extract（part Ⅰ）,ethyl acetate extract（part Ⅱ）,butanol extract water saturated butanol extract（part Ⅲ） and residual water layer extract（part Ⅳ） of the Xiao Chaihu decoction.The treatment groups received part Ⅰ-Ⅳ at dose of 9.0,18.0 g.kg-1 and fluoxeine at 20 mg.kg-1 for 7 days respectively.The tail suspension test（TST） and forced swimming test（FST） were carried out to compare the antidepressant effects of different fractions from Xiao Chaihu decoction at 1 hour after the last administration.Result： The group of part Ⅱ and part Ⅲ could significantly shorten mice immobility time in FST and TST（P 0.05 or P 0.01）.Conclusion： The antidepressant pharmacodynamic tests shows that ethyl acetate extract and butanol extract can be initially determined as the antidepressant effective fraction of Xiao Chaihu decoction.