桑叶黄酮对四氯化碳诱导的小鼠急性肝损伤保护作用

Protection Effects of Flavonoids from Morus alba Leaves on Carbon Tetrachloride Induced Liver Injury in Mice

目的:探讨桑叶黄酮对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法:小鼠随机分为空白组,模型组,联苯双酯100 mg.kg-1组、桑叶黄酮200,400,800 mg.kg-1组,连续ig给药14 d,1次/d。电镜观察CCl4急性肝损伤模型小鼠肝组织形态学变化,分光光度法测定小鼠血清中门冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT),肝组织中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量。结果:桑叶黄酮能显著降低CCl4所致小鼠血清ALT和AST活性的升高(P<0.01),并能显著升高SOD和GSH-Px活性(P<0.01),降低MDA含量(P<0.01),保护肝细胞。结论:桑叶黄酮对CCl4诱导的小鼠肝损伤有很强的保护作用。

Objective： To study the protection effects of Flavonoids extraction from Morus alba leaves（FE） on carbon tetrachloride（CT） induced liver injury mice and investigate the possible mechanisms.Method： Kunming mice were randomized into six groups（n = 10,each）,The blank group,the model group,bifendate group（100 mg.kg-1）,high dose of FE group（200 mg.kg-1）,middle dose of FE group（400 mg.kg-1） and low dose of FE group（800 mg.kg-1）.Mice were orally administered corresponding drugs one time daily for 14 days,the same volume of saline was infused in blank group and model group.The treated groups and model group were ip given 0.15% CCl4 in peanut oil（0.01 mL.g-1） once to produce acute liver injury on the 15th day.The morphological changes of the liver tissue were oberserved by HE stain.the serum activity of alanine transaminase（ALT）,aspartate transaminase（AST） and the activity of superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px） and content of malondialdehyde（MDA） in liver were detected by spectrophotometry.Result： Compraed with the model group,the activity of ALT,AST and content of MDA in serum were obviously decreased（P 0.01）,the activity of SOD,GSH-Px were increased markedly（P 0.01）.Conclusion： FE has protective effects against acute liver injury induced by CCl4 in mice.