化瘀通痹胶囊治疗风湿性关节炎的实验研究

An Experimental Study on Effect of Huayu Tongbi Capsule for Rheumatic Arthritis

目的:考察化瘀通痹胶囊对风湿性关节炎的治疗作用。方法:给予大鼠Freund’s完全佐剂复制佐剂性关节炎模型。实验分为空白对照、模型对照(等容去离子水)、寒湿痹颗粒(3.5 g.kg-1)组与化瘀通痹胶囊高、中、低剂量(生药,1.1,0.55,0.27 g.kg-1)组,ig每天1次,连续14 d,实验期间于造模后第3,6,8,11,14,18,21,24天测量大鼠致炎足踝关节周长,第8,11,14,18,21,24天测量致炎对侧足踝关节周长,并于18,21 d测定致炎足机械性压迫所致的痛阈值;通过蛋清致大鼠足肿胀实验、二甲苯致小鼠耳肿胀实验、小鼠扭体实验,考察受试药物的抗炎、镇痛作用。实验分为空白对照、寒湿痹颗粒组与化瘀通痹胶囊高、中、低剂量组,大鼠给药同佐剂性关节炎实验,小鼠给药为空白对照、寒湿痹颗粒(4.5 g.kg-1)组与化瘀通痹胶囊高、中、低剂量(1.4,0.7,0.35 g.kg-1),每天1次,连续ig 7 d。结果:与模型组比较,化瘀通痹胶囊高、中剂量组均改善佐剂性关节炎模型大鼠致炎足18,21 d踝关节周长(P<0.05),高剂量组可显著提高致炎18,21 d大鼠机械痛阈值(P<0.05);中剂量组可显著提高致炎21 d大鼠机械痛阈值(P<0.05);与模型对照组比较,化瘀通痹胶囊高、中剂量组可抑制蛋清致炎后4h大鼠足跖肿胀度(P<0.05);高剂量组能降低二甲苯致小鼠耳肿胀的程度(P<0.05);高、中剂量组小鼠扭体反应次数明显减少(P<0.05)。结论:化瘀通痹胶囊具有良好的抗炎、镇痛作用,对风湿性关节炎具有明显的预防和治疗作用。

Objective： To study the effect of Huayu Tongbi capsule therapy treating rheumatic arthritis.Method： Induce the model of arthritis in rats by Freund’s complete adjuvant（FCA）.The rats were used and randomly divided into 6 groups： blank control,model control（isovolumic deionized water）,Hanshibi granule（3.5 g.kg-1） and low dosage,middle dosage and high dosage with Huayu Tongbi capsule（1.1,0.55,0.27 g.kg-1）.Drugs were administered to animals by gastric perfusion once a day for 14 days.During the experiment,measured the paw swelling of inflammation rats at 3,6,8,11,14,18,21,24 d,measured the opposite paw swelling of inflammation rats 8,11,14,18,21,24 d and measured the pain threshold value of rat by mechanical compression at 18,21 d.To study the anti-inflammatory and analgesic effects of drug by the mean of the ear edema resulted from dimethyl benzene,paw edema induced by egg white and mice twisting test.The experiment of rats same to above,The mouse were used and randomly divided into 5 groups： model control（isovolumic deionized water）,Hanshibi granule（3.5 g.kg-1） and low dosage,middle dosage and high dosage with Huayu Tongbi capsule（1.4,0.7,0.35 g.kg-1）,once a day for 7 days.Result： Compared to model group,the results degree of the paw swelling of inflammation rats were improved in the middle dosage and high dosage with Huayu Tongbi Capsule at 18,21 d（P 0.05）,the results degree of the pain threshold value of rats by mechanical compression were improved in the high dosage with Huayu Tongbi capsule at 18,21 d（P 0.05）.Compared to blank group,the paw swelling of inflammation rats by egg white were decreased significantly in the middle dosage and high dosage with Huayu Tongbi capsule at 4 h（P 0.05）,the ear edema resulted from dimethyl benzene was decreased in the high dosage（P 0.05）,the mice twisting test were decreased significantly in the middle dosage and high dosage（P 0.05）.Conclusion： Huayu Tongbi capsule has the effect of anti-inflammatory and analgesia,can prevent and treat the Rheumatic arthritis.