期刊文献+

手足口病患儿外周血细胞因子及T淋巴细胞检测结果分析 被引量:3

Test of serum cytokines and lymphocytes in children with hand, foot-and mouth disease
下载PDF
导出
摘要 目的检测手足口病患儿外周血细胞因子和免疫T淋巴细胞变化,并与正常儿童对比,探讨其免疫学发病机制。方法细胞因子(白介素6、10、17、21、23)检测采用酶联免疫吸附(ELISA)法,免疫T淋巴细胞(CD3+、CD3+CD4+、Treg细胞、Th17细胞)检测采用流式细胞仪。结果①手足口病患儿白介素6、10、17、21、23结果均升高,分别是(40±0.8)、(54±1.1)、(33±0.6)、(33±0.9)和(27±0.8)pg/L;正常小儿对照组分别为(17±1.0)、(23±0.6)、(19±0.8)、(21±0.6)和(19±0.8)pg/L,两组比较,差异有统计学意义(P<0.05)。②免疫T淋巴细胞CD3+、CD3+CD4+、Treg细胞、Th17细胞分别为(44±0.9)%、(27±0.7)%、(20±0.4)%、(28±1.0)%;正常小儿对照组分别为(52±1.1)%、(44±1.1)%、(24±0.7)%、(3.6±0.5)%,Th17细胞升高,两组比较,差异有统计学意义(P<0.05)。结论手足口病患儿急性期白介素升高,Th17细胞升高,存在免疫系统功能紊乱。 [ Objective ] To investigated the changes and significances of cyterkines and lymphocytes in children with hand, foot and mouth disease(HFMD). [ Methods ] Fifty nine cases with HFMD and thirty healthy children as controls were enrolled in study. The percentage of lymphocyte subsets(including CD3+, CD3+CD4+, Treg and Thl7 cells) and cyterkines(including interleukin 6,10,17,21,23) in two groups were determined by four color fluorescence flow cytometry and ELISA. [ Resluts ] Compared with normal controls, the HFMD patients showed an obvious decrease in percentage of CD3+, CD3+ CD4+,Treg cells, but obvious elevation of Thl7cells and cyterkines. Significant difference was found in two groups. [ Conclusion ] There are functional disorders in cellular and humoral immunity in HFMD patients, Abnormal immunity may play an important role in the children with HFMD.
出处 《中国妇幼卫生杂志》 2013年第3期1-1,4,共2页 Chinese Journal of Women and Children Health
基金 广州市医药卫生科技项目(201102A213091)
关键词 手足口病 细胞因子类 淋巴细胞 hand, foot-and mouth disease cytokines lymphocytes
  • 相关文献

参考文献5

二级参考文献32

  • 1瞿少刚,张秀萍.病毒性脑膜炎、脑炎患儿的病原分析及部分生化指标变化的临床意义[J].现代医药卫生,2006,22(6):798-800. 被引量:11
  • 2何时军,陈贤楠.病毒相关性小儿危重病[J].中国小儿急救医学,2006,13(1):72-74. 被引量:37
  • 3包玉梅.病毒性脑炎89例脑脊液分析[J].现代医药卫生,2007,23(4):533-534. 被引量:10
  • 4周浩泉,吕勇,潘家华.小儿不典型结核性脑膜炎46例临床分析[J].实用全科医学,2007,5(1):35-36. 被引量:6
  • 5Schmidt N J, Lennette E H, Ho H H. An apparently new enterovirus isolated from patients with diseases of the central nervous system [ J ]. J Infect Dis, 1974, 129:304 - 309.
  • 6Ho M, Chen E R, Hsu K H, et al. The enterovirus type 71 epidemic of Taiwan, 1998 [J]. N Engl J Med, 1999, 341:929- 935.
  • 7郑志明 张江虹 诸卫平.从我国成人手足口病患者中首次分离出肠道病毒71型.武汉大学学报:医学版,1989,1:27-29.
  • 8Liu C C, Tseng H W, Wang S M, et al. An outbreak of enterovirus 71 in Taiwan, 1998: epidemiologic and clinical manifestation [J]. J Clin Virol, 2000, 17:23 -30.
  • 9Wang S M, Liu C C, Tseng H W,et al. Clinical spectrum of enterovirus 71 infection in children in southern Taiwan, with an emphasis on neurological complication [ J ]. Clin Infec Dis, 1999,19 : 184 - 190.
  • 10Chang L Y, Lin T Y, Hsu K H, et al. Clinical features and risk factors of pulmonary oedema after enterovirus-71-related hand, foot, and mouth disease[J]. Lancet, 1999, 354:1682 -1"686.

共引文献136

同被引文献20

引证文献3

二级引证文献11

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部