手足口病患儿外周血细胞因子及T淋巴细胞检测结果分析

Test of serum cytokines and lymphocytes in children with hand, foot-and mouth disease

目的检测手足口病患儿外周血细胞因子和免疫T淋巴细胞变化,并与正常儿童对比,探讨其免疫学发病机制。方法细胞因子(白介素6、10、17、21、23)检测采用酶联免疫吸附(ELISA)法,免疫T淋巴细胞(CD3+、CD3+CD4+、Treg细胞、Th17细胞)检测采用流式细胞仪。结果①手足口病患儿白介素6、10、17、21、23结果均升高,分别是(40±0.8)、(54±1.1)、(33±0.6)、(33±0.9)和(27±0.8)pg/L;正常小儿对照组分别为(17±1.0)、(23±0.6)、(19±0.8)、(21±0.6)和(19±0.8)pg/L,两组比较,差异有统计学意义(P<0.05)。②免疫T淋巴细胞CD3+、CD3+CD4+、Treg细胞、Th17细胞分别为(44±0.9)%、(27±0.7)%、(20±0.4)%、(28±1.0)%;正常小儿对照组分别为(52±1.1)%、(44±1.1)%、(24±0.7)%、(3.6±0.5)%,Th17细胞升高,两组比较,差异有统计学意义(P<0.05)。结论手足口病患儿急性期白介素升高,Th17细胞升高,存在免疫系统功能紊乱。

[ Objective ] To investigated the changes and significances of cyterkines and lymphocytes in children with hand, foot and mouth disease（HFMD）. [ Methods ] Fifty nine cases with HFMD and thirty healthy children as controls were enrolled in study. The percentage of lymphocyte subsets（including CD3＋, CD3＋CD4＋, Treg and Thl7 cells） and cyterkines（including interleukin 6,10,17,21,23） in two groups were determined by four color fluorescence flow cytometry and ELISA. [ Resluts ] Compared with normal controls, the HFMD patients showed an obvious decrease in percentage of CD3＋, CD3＋ CD4＋,Treg cells, but obvious elevation of Thl7cells and cyterkines. Significant difference was found in two groups. [ Conclusion ] There are functional disorders in cellular and humoral immunity in HFMD patients, Abnormal immunity may play an important role in the children with HFMD.