MicroRNA-144在结肠癌中的表达水平及临床意义

Clinical significance of microRNA-144 expression in colon tumor

目的探讨microRNA-144在结肠癌中的表达水平及其与临床病理特征的相关性。方法提取38例结肠癌及其正常结肠黏膜组织标本的总RNA,采用逆转录实时荧光定量聚合酶链反应(RT-qPCR)方法检测microRNA-144的表达量,并分析其与临床分期、分化程度、淋巴结转移等临床病理特征的关系。结果癌与癌旁正常结肠黏膜组织比较,microRNA-144在癌组织中高表达,相对表达量的中位数是2.30。其表达与TNM分期(P<0.05)、分化程度(P<0.05)相关,受试者工作特征(ROC)曲线分析显示,以相对表达量1.70为临界点时,microRNA-144作为评价结肠癌细胞分化程度,敏感性为88%,特异性为61.5%。结论 microRNA-144在结肠癌中的异常表达可能与结肠癌的发生、发展有关。

Objective To investigate the expression of microRNA-144 and its association with clinic-pathological features in colon tumor.Methods The expression levels of microRNA-144 in 38 colon tumors and their matched non-tumor adjacent tissue specimens were detected by using stem-loop real-time reverse transcription-polymerase chain reaction（RT-qPCR）,and their correlation with clinic-pathologic features of colon tumor was analyzed.Results Expression levels of microRNA-144 were significantly higher in tumor tissues than in adjacent normal tissues（P0.05）,the median of comparative expression level was 2.30.The expression of microRNA-144 was associated with TNM stage（P0.05）and cell differentiation（P0.05）.ROC curve of microRNA-144 about cell differentiation and resulted in a diagnostic sensitivity of 88% and specificity of 61.3%.Conclusion The level of microRNA-144 expression may be a valuable adjuvant parameter in forecasting the poorly differentiated colon tumor.