不同时间给予大红袍水提物对小鼠急性酒精性肝损伤的影响

Influence of Dahongpao Tea Water Extract Feeding at Different Time on Alcohol-induced Acute Liver Injury in Mice

为了比较提前2 h给予、同时给予及推后2 h给予大红袍水提物对小鼠急性酒精性肝损伤的抵御效果,将50只小鼠随机分成5组:对照组给予等量的蒸馏水,乙醇组一次性给予60%乙醇(0.01 mL/g),提前组、同时组及推后组分别在灌喂酒精前2 h、灌喂酒精中和灌喂酒精后2 h给予大红袍水提物。通过检测小鼠体重、肝重以及血清及肝匀浆中谷草转氨酶(AST)、谷丙转氨酶(ALT)、总胆固醇(TC)、甘油三酯(TG)、丙二醛(MDA)、总巯基等相关指标,并采用HE染色观察肝组织脂肪变性程度。结果表明,一次性豪饮乙醇的急性酒精性肝损伤小鼠模型造模成功,小鼠肝脏组织细胞、血清的生化代谢指标、抗氧化指标及显微观察结果都显示,提前组、同时组和推后组均能不同程度地抵御小鼠急性酒精性肝损伤,以提前组效果最佳,同时组次之,推后组效果不太明显。

In order to compare the effect of Dahongpao tea water extract feeding at different time on alcohol-induced acute liver injury in mice. Fifty mice were randomly divided into five groups and were intragastrically given 60% ethanol （0.01 mL/g） alcohol （alcohol group） or distilled water （normal control）. Dahongpao tea water extract was fed at different time, including feeding 2 hours before drinking, feeding while drinking and feeding 2 hours after drinking alcohol. All mice were sacrificed to take serum samples for biochemical measurements, such as glutamic oxalacetic transaminase （AST）, glutamic-pyruvic transminase （ALT）, total cholesterol （TC）, total triglyceride （TG）, malondialdehyde （MDA） and total sulfydryl, liver samples for microexamination using HE staining to determine the degree of fatty changes. A mice model of alcohol induced acute liver injury has been successfully established biochemicalmetabolism index, antioxidant index and microexamination results in mice liver showed that Dahongpao tea water extract fed at different time is able to protect mice from alcohol-induced acute liver injury in different degree. Fed 2 hours before drinking is of best protective effect, and fed while drinking is the second, However, the effect of fed 2 hours after drinking is not significant.