潜阳解毒通络饮通过PPAR-γ途径抑制炎症因子表达从而对自发性高血压大鼠炎症机制干预的研究

Qianyang Jiedu Tongluo Decoction on inflammatory intervention mechanism in spontaneously hypertensive rats expression by acting on PPAR-γ pathway inhibition of inflammatory factors

目的探讨潜阳解毒通络饮治疗原发性高血压大鼠的作用机制。方法选择健康雄性12周龄原发性高血压大鼠(spontaneously hypertensive rats,SHR)71只,随机分为潜阳组、解毒组、通络组、全方组、缬沙坦组、吡格列酮组、SHR空白对照组,魏-凯二氏大鼠(Wistar-Kyoto rats,WKY)大鼠10只作为正常血压对照组。给药2个月后处死取腹主动脉血和心脏左心室。用ELISA试剂盒测血清中超敏C反应蛋白(high-sensitivity c-reactive protein,hs-CRP)、肿瘤坏死因子(tumor necrosis factor-a,TNF-a)、白介素1β(interleukin-lβ,IL-1β)的浓度。聚合酶链反应(polymerase chain reaction,PCR)测过氧化物酶体增殖物激活受体γ(peroxisome proliferators activated receptors,PPAR-γ)、果蝇受体4(toll-like receptor4,TLR4)及TNF-a的mRNA在大鼠心肌细胞中的表达。结果潜阳解毒通络饮能够有效抑制SHR血清中IL-1β、TNF-α及hs-CRP炎症因子的表达,有统计学意义(P<0.05)。在SHR心肌组织mRNA的表达上,TNF-a、TLR4及PPAR-γ的mRNA表达与其他治疗组和对照组相比有统计学意义(P<0.05)。结论证实了潜阳解毒通络饮从"风痰瘀络"病机的角度治疗原发性高血压大鼠有效性,其作用机制可能与作用于PPAR-γ途径、改善炎症因子表达有关。

Objective To investigate the mechanism of Qianyang Jiedu Tongluo Decoction for the treatment of hypertensive rats. Methods healthy male 12 weeks Lingyuan hypertension rat （SHR） 72, 10 WKY rats, randomly divided into Qianyang Group, detoxifieation group, Tongluo group, the whole Party group, valsartan group, SHR treatment group, blank control group and Control group. After 2 months of administration take blood from the abdominal aorta and the left ventricle of the heart. The concentration of serum hs-CRP, TNF-a, IL-1β ELISA kit. Expression of PPAR-γ, TNF-a and TLR4 RT-PCR mRNA in myocardial cells of rats. Results Qianyang Jiedu Tongluo Decoction can effectively inhibit the expression of SHR in rat serum IL-1β, TNF-α and hs-CRP inflammatory factors, with statistical significance （ P 〈 0. 05 ）. The expression of mRNA in myocardial tissue of SHR rats on the TNF-α, TLR4, and PPAR-γ mRNA expression compared with other treatment group and the control group was statistically significant（ P 〈0. 05 ）. Conclusion the treatment of confirmed hypertensive rats from the ＂wind phlegm blood sta- sis collaterals＂ pathogenesis angle Qianyang Jiedu Tongluo Decoction is effective, its mechanism may be re- lated to effects on PPAR-γ way, improve the expression of inflammatory factors relat.