小鼠肾脏纤维化Masson染色方法的改进

Improvement of Masson staining in renal fibrosis of mice

目的改进Masson三色染色法,以获得较好显示肾脏纤维化的染色方法。方法用Bouin液补充固定组织切片;用-20℃储存的乙醇∶乙酸(2∶1)溶液处理组织;用亮绿代替苯胺蓝;减少亮绿的染色时间。结果明确区分纤维化肾间质和未纤维化的肾间质,胶原纤维呈绿色(用亮绿复染),胞质和肌纤维呈红色。结论改进后的Masson方法可以将纤维化和非纤维化的肾脏组织进行明显的区分,显微镜下结构显示清晰,着色均匀,取得了较为满意的显色对比效果。

Objective To get an improved Masson 3 staining method for staining renal fibrosis. Methods Bouin＇s fluid was used to re-fix the paraffin fixed sections. Ethanol： acetic acid（2：1 ） which was stored at -20℃ was used to treat the sections; Brilliant green was used to substitute aniline blue. Duration of brilliant green staining was decreased. Results Renal interstitium of fibrosis and non-fibrosis were distinguished. Collagen fibers appeared green （counter staining by brilliant green） and cytoplasm and muscle fibers were red. Conclusion In improved Masson 3 staining, tissues of fibrosis and non-fibrosis are distinguished by brilliant green, the staining results are stable and uniform. The method demonstrates a satisfied compared staining result.