维甲酸相关核孤儿受体γt和4-1BB/4-1BBL在口腔扁平苔藓中的表达及意义

Expression and significance of retinoid-related orphan receptor γt and 4-1BB/4-1BBL in oral lichen planus

目的研究口腔扁平苔藓(OLP)患者外周血中维甲酸相关核孤儿受体γ(tRORγt)和4-1BB/4-1BBL mRNA的表达。方法采用实时荧光定量聚合酶链反应(FQ-PCR)法检测30例OLP患者和30例健康人外周血中RORγt和4-1BB/4-1BBL的基因表达水平。结果在OLP患者中,RORγt、4-1BB、4-1BBL mRNA的表达水平显著高于对照组(P<0.05),结果经2-ΔΔCT转换后,OLP组3种基因mRNA表达水平分别是健康对照组的3.087、3.320和4.005倍。RORγt和4-1BB mRNA的表达水平无明显相关性(P>0.05),4-1BB和4-1BBL mRNA表达水平呈正相关(r=0.455 0,P<0.05)。结论RORγt和4-1BB/4-1BBL在OLP的发病中起了一定作用。

Objective To investigate the expression of the peripheral blood retinoid-related orphan receptor γt （RORγt） and 4-1BB/4-1BBL mRNA in oral lichen planus（OLP） patients. Methods The expression levels of peri- pheral blood＇ RORγt mRNA and 4-1BB/4-1BBL mRNA of 30 samples of OLP patients and 30 cases of healthy hu- man were detected using real-time fluorescent quantitative-polymerase chain reaction （FQ-PCR） technology. Results In the OLP group, the expression level of RORγt, 4-1BB, 4-1BBL mRNA were higher than those in the control group （P〈0.05）. After the conversion of the 2-△△CT, the expression levels of three kinds of mRNA in the OLP group were 3.087, 3.320 and 4.005 times of the control group respectively. In the OLP group, the expression levels of RORγt mRNA and 4-1BB mRNA had no obvious correlation（P〉0.05）, the expression levels of 4-1BB and 4-1BBL mRNA had a positive correlation （r=0.455 0, P〈0.05）. Conclusion The abnormal expression of RORγt, 4-IBB and 4-1BBL mRNA may play certain roles in the pathogenesis of OLP.