皖西白鹅换羽前后基因表达谱差异分析

Identification of Differentially Expressed Genes in Skin of Wanxi-white Goose during Regeneration of Downy Feather

旨在研究影响羽绒再生过程关键基因的表达调控。本试验选取6只100日龄皖西白鹅母鹅,于换羽前和换羽后1d分别取其皮肤样,提取RNA,等量混合后比较换羽前后皖西白鹅差异表达基因。结果表明,Wnt信号通路中的wnt5a、wnt10a及Lipoprotein receptor-related protein 3(LRP3)均表达下调,wnt受体基因frizzled 2(FZD2)、FZD9、SFRP2表达上调,而FRZB、FZD8、FZD10表达下调;Noggin途径的NOG2和NOG均表达下调,BMP家族的BMPR1A和BMP1表达上调,BMP2、BAMPI、BMP2K及BMP7等基因均表达下调;涉及羽绒再生循环的其他相关基因如细胞周期相关蛋白基因Cyclin B3和Cyclin Y表达上调,而Cyclin D3和CDK3表达下调;皖西白鹅羽绒再生过程中还检测到类胰岛素生长因子IGF2、转化生长因子IGFα、白介素家族IL6ST等、肿瘤坏死因子TNFSF15等基因差异表达。皖西白鹅换羽1d后引起Wnt经典途径和Noggin路径等多条信号通路上相关基因的差异表达。通过对皖西白鹅换羽前后基因表达差异分析,为进一步丰富禽类羽绒再生分子机理提供重要信息,羽绒再生过程关键调控基因的筛选将为鹅高产羽绒品系的选育提供依据。

The aim of this study was to exploit genes that play key roles during feather regeneration. In this experiment, 6 female Wanxi-white geese with 100-day-old were selected for skin samples before and 1 d after moulting. The 6 RNA samples were equally mixed for detecting the differentially expressed genes before and after moulting in Wanxi-white goose. The results showed that Wnt5a, WntlOa and Lipoprotein receptor-related protein 3 （LRP3） genes in Wnt signaling pathway were down-regulated, and wnt receptors genes （frizzled 2 （FZD2）, FZD9 and SFRP2） were up-regulated while FRZB, FZD8 and FZDIO were down-regulated 1 d after moulting in Wanxi-white goose. In the Noggin signaling pathway, NOG2 and NO（; were down- regulated, and BMPRIA and BMP1 were up-regulated while BMP2, BAMPI, BMP2 and BMP7 were down-regulated 1 d after moulting. Cell cycle-related protein Cyclin B3 and Cyclin Y were up-regulated while Cyclin D3 and CDK3 were down-regulated. Other genes, such as IGF2, IGFa, IL6ST, TNFSF15, were also detected as differentially expressed genes 1 d after moulting in Wanxi-white goose. Many genes which participated in Wnt signaling pathway and Noggin signaling pathway, were detected in differential expression 1 d after moulting in Wanxi-white goose.The differentially expressed genes detected in this experiment would provide more information in the molecular regulation of downy feather regeneration. Genes screened from this experiment might be taken as key regulatory genes for breeding of goose with high-yield downy feather.