电刺激大鼠皮层桶状区对胆碱乙酰移位酶表达的影响

Expression of choline acetyltransferase in the rat barrel cortex by electrical stimulation

目的:观察电脉冲刺激大鼠大脑皮层桶状区(BC)诱导的转向运动,并观察电刺激后胆碱乙酰移位酶(ChAT)在大鼠BC区的表达。方法:将大鼠随机分为3组:空白组、对照组、实验组。对照组和实验组大鼠在双侧BC区埋植刺激电极;术后7 d实验组大鼠给予电刺激诱导左右转向运动;连续刺激3 d后,取脑行冰冻切片常规HE染色和免疫组织化学染色;并提取3组大鼠海马和脑组织蛋白,用Western blot方法检测ChAT的表达。结果:给予电脉冲刺激大鼠BC区,实验动物均出现向左或向右转;HE染色显示电极周围组织没有明显形态学变化。与对照组和空白组相比,免疫组化和Western blot结果均显示实验组大鼠BC区ChAT阳性的表达率增高。结论:电刺激BC区可诱导大鼠产生特定的方向性运动;且大鼠脑组织中ChAT表达增加,提示胆碱能神经递质参与了感觉-运动皮层之间的信息传递。

Objective： To observe a turning performance in the rats excited by using a proper electrical stimuli of the barrel cortex region（BC）,and the expression of choline acetyltransferase（ChAT） in the BC regions after electoral stimulation.Methods： SD rats were divided into three groups.The stimulation electrodes were surgically implanted into the bilateral BC regions in the control group and the experimental group rats.The experiment group post surgery for seven days was given the electrical impulses via connection with the electrodes for three times each day through consecutive three days.Three groups of the rats were killed and the brains were quickly removed for frozen sections and then performed with conventional HE and immunohistochemistry staining.And protein samples were prepared from brain and the hippocampus tissues of the three groups to detect the level of the ChAT protein by Western blot.Results： The experimental rats turn left or right when continuously stimulation in the bilateral BC regions with electric pulse.HE staining showed no significant damage around electrodes in the cerebral cortex.Compared with the control and blank groups,the ChAT positive rate in the brain section in the experimental rats was significantly high by immunohistochemistry assay;the level of the ChAT protein in the rats given the electrical stimulation increased.Conclusion： Turnings performance of the rat could be initiated by electrical stimuli in the BC region.Expression of ChAT is significantly higher in the BC regions of rat under electrical stimulation,suggesting that acetylcholine might be associated with signal transmission between senses and movement behavior in the nervous central system.