复方中药制剂对电磁辐射致大鼠海马神经损伤的防治作用

The Chinese medicine nutrient diet intervention prevent against the neurologic damage induce by EMF irradiation in rat hippocampus

目的:探讨含阿魏酸、黄芪多糖和红景天甙三种中药成分的复方中药剂对电磁辐射致中枢神经损伤的保护作用。方法:大鼠80只随机分为10组,分为普通饲料喂养组共5组,普通饲料假辐照组、普通饲料慢性辐照组、普通饲料急性辐照3 h组、24 h组、72h组;复方中药制剂组共5组,分复方中药制剂假辐照组、复方中药制剂慢性辐照组、复方中药制剂急性辐照3 h组、24 h组、72 h组。急性辐照采用65 W/cm2一次辐照20 min,慢性辐照采用15 W/cm2每天辐照20 min,连续8周,复方中药制剂组在普通饲料喂养基础上采用复方中药每天灌胃。采用Morris水迷宫测试大鼠学习记忆能力,TUNEL染色观察海马神经细胞凋亡,生化检测丙二醛(MDA),超氧化物歧化酶(SOD),活性氧(ROS),谷胱甘肽过氧化物酶(GSH-Px)。结果:电磁辐射急、慢性辐照后大鼠学习记忆能力明显下降(P<0.05),海马神经细胞凋亡率增加(P<0.05),海马SOD及GSH-PX活性下降、ROS及MDA含量增加(P<0.05)。与普通饲料组比较,复方中药试剂干预组能使急性辐照后24 h学习记忆能力明显恢复(P<0.05);急性辐照72 h组和慢性辐照组细胞凋亡显著减少(P<0.05);并显著增加急慢性辐照后SOD、GSH-PX活性(P<0.05),减少ROS、MDA含量(P<0.05)。结论:复方中药制剂能有效保护电磁辐射对学习记忆神经行为功能的影响,减少海马神经细胞凋亡,维持和恢复大鼠海马脑组织的氧化还原平衡,且这种神经保护作用在电磁辐射急性辐照后期以及慢性辐照中更为明显。

Objective： To observe the neurologic damage in rat hippocampus after electromagnetic field（EMF） acute or chronic irradiation and research the protective effects of Chinese medicine diet（CMD） which comprised ferulic acis,ginsenoside,astragalus polysaccharide and rhodiola sachaliensis.Methods： Eighty rats were divided into ten groups（n=8）： normal diet with shame irradiatin group（NS）,normal diet with chronic irradiation group（NCI）,three groups of normal diet with acute irradiation after 3 h,24 h,72 h（NAI）,Chinese medicine diet with shame irradiatin group（CS）,Chinese medicine diet with chronic irradiation group（CCI）,three groups of Chinese medicine diet with acute irradiation after 3 h,24 h,72 h（CAI）.The chronic EMF irradiation were performed by electromagnetic wave at 15 W/cm2 for 20 min everyday for 8 weeks continuously.The acute EMF irradiation were performed by electromagnetic wave at 65 W/cm2 for 20 min after feeding with CMD for 8 weeks.The learning and memory were evaluated by Morris water maze before/after electromagnetic wave irradiation.The apoptotic cells in hippocampus was detected by Tunel staining.The peroxidation damage of EMF and the protective effect of CMD intervention were assaied by measuring superoxide dismutase（SOD）、malondialdehyde（MDA）、glutathione peroxidase（GSH-Px） and reactive oxygen species（ROS）.Results： The acute and chronic EMF irradiation disturbed the ability of learning and memory significantly（P＆lt;0.05）,CMD intervention markedly antagonized this effect.The apoptotic cells in hippocampus increased evidently after EMF irradiation（P＆lt;0.05）,but CMD intervention reduced the apoptotic cells.The acute and chronic EMF irradiation induced the oxidative stress by down-regulating SOD activity,GSH-Px activity,ROS inhibiting and up-regulating the content of MDA obviously（P＆lt;0.05）,and CMD intervention reduced peroxidation damage significantly（P＆lt;0.05）.Conclusion： The acute and chronic EMF irradiation could initiate neurologic damage in hippocampus.CMD intervention has protective effect on the impaired learning and memory,the neuron apoptosis,the peroxidation damage induced by EMF irradiation.CMD intervention plays a significant protective role in antagonizing neurologic damage in the later stage of acute irradiation and chronic irradiation.