颜面播散性粟粒性狼疮患者外周血T淋巴细胞AgNORs的检测

The Clinical Significance of Detecting the T-AgNORs in the Peripheral Blood of Patients with LMDF

目的观察颜面播散性粟粒性狼疮(LMDF)患者外周血T淋巴细胞核仁区嗜银蛋白染色(AgNORs)情况和应用氨苯砜和转移因子联合治疗前、后患者免疫功能变化情况。方法对30例LMDF患者治疗前、后外周血T淋巴细胞AgNORs进行检测,同时予氨苯砜及转移因子注射液联合治疗3个月后观察疗效,并以30例健康人为对照。结果 LMDF患者治疗前AgNORs的测定值[(6.35±0.45)%]明显低于正常对照组[(7.85±0.49)%]和其治疗后[(7.74±0.46)%],差异均有统计学意义(P均<0.05)。治疗3个月后的临床疗效好于治疗2个月时。结论 AgNORs测定可以反映LMDF的免疫状况,氨苯砜联合转移因子治疗LMDF疗效较好。

Objective The study tried to observe the patients immune function and to compare the immune function be- fore and after treatment by detecting peripheral blood T lymphocytes nucleolus AgNOR staining （AgNORs）. Methods Firstly, peripheral blood T lymphocytes AgNORs were detected and analyzed in 30 LMDF pa- tients before and after treatment, then they were compared with 30 healthy controls; Secondly, the effect of combination therapy with dapsone and transfer factor on 30 cases LMDF patients were observed for three months. Results The value of T lymphocyte AgNORs of LMDF patients [ （6.35 ± 0.45 ） % ] before treat- ment was lower significantly than that of healthy controls [ （7.85 ± 0.49） % ], and that of LMDF patients after treatment [ （7.74 ± 0.46） % ]. Both the differences have statistical significance （ all P 〈 0.05 ）. The treatment effect of 3 months was better than that of 2 months. Conclusion AgNORs can reflect the immune status of the LMDF patients. The therapy combined dapsone with transfer factor for LMDF have better effect.