表没食子儿茶素没食子酸酯对人成淋巴细胞株hMLH1和hMSH2 mRNA表达的影响

Effects of (-)epigallocatechin gallate on expressions of hMLH1 and hMSH2

目的:探讨表没食子儿茶素没食子酸酯[(-)epigallocatechin gallate,EGCG]对人成淋巴细胞株错配修复基因hMLH1和hMSH2 mRNA表达水平的影响。方法:将正常人成淋巴细胞株GM12593和乳腺癌患者成淋巴细胞株GM13705分别置于含有0、5、10、20μmol/LEGCG的RPMI-1640中进行6d干预培养后,采用实时荧光定量PCR(FQ-PCR)技术检测干预前后错配修复基因hMLH1和hMSH2 mRNA表达水平的变化。结果:经20μmol/LEGCG干预培养6d后,GM12593细胞hMLH1与hMSH2 mRNA表达水平均显著高于其他浓度组(P均<0.05),且显著高于同等浓度时GM13705细胞中上述2个基因的表达水平(P<0.05);EGCG对GM13705目标基因的表达无显著影响(P>0.05)。结论:EGCG具有上调正常人成淋巴细胞株hMLH1与hMSH2 mRNA表达水平的潜力,可能通过增加错配修复起始复合物的数量,来帮助错配修复机制的启动,维护基因组的稳定性。

OBJECTIVE： To investigate the effect of （-） epigallocatechin gallate（EGCG） on mRNA expressions of hMLH1 and hMSH2 in human lymphoblast cell lines. METHODS： Total RNA was isolated from GM12593 and GM13705 ceils treated with EGCG（0, 5, 10, 20 μmol/L） for 6 days. Real-time fluorecence quantification PCR（FQ-PCR） was conducted to measure the mRNA expression levels of hMLH1 and hMSH2. RESULTS： The mRNA expressions of hMLH! and hMSH2 significantly increased at 20 μmol/L EGCG in GM12593 cells, and were significantly higher than equal concentrations in GM 13705 cells. The effect of EGCG on expressions of target genes were insignificant in GM13705 ceils. CONCLUSION： EGCG had the potential to up-regulate the expressions of hMLH1 and hMSH2, contributed to the promotion of mismatch repair and maintaining genomic stability in normal human lymphoblast cell line GM12593.