二甲双胍和辛伐他汀联用药对糖尿病大鼠骨密度变化的实验研究

Experimental study of the effect of metformin combined with simvastatin on the change of bone mineral density in diabetic rats

目的建立糖尿病大鼠动物模型,观察使用二甲双胍和辛伐他汀对胫骨骨密度的影响,为临床糖尿病患者种植体修复、正畸治疗提供理论依据。方法采用空腹一次性注射STZ溶液(40 mg/kg),注射STZ 3天后,大鼠空腹血糖高于16.7 mmol,尿糖(+++),视为糖尿病大鼠模型建立成功。建模后,分正常组,模型组,二甲双胍组,二甲双胍和辛伐他汀联用组,检测胫骨骨密度。结果糖尿病大鼠胫骨骨密度低于二甲双胍组低于二甲双胍和辛伐他汀联用组低于正常组(P<0.01)。结论糖尿病状态下胫骨骨密度低于正常,经过治疗后骨密度改善明显,联用组优于单独使用二甲双胍组。

Objective To establish diabetic rat model, to observe the effect of metformin combined with simvastatin on the change of bone mineral density （BMD） of the tibia, in order to provide a theoretical basis for implant treatment and orthodontic treatment for patients with diabetes. Methods Fasting rats were given a single injection of STZ solution （40 mg/kg）. Three days after the injection, the fasting blood sugar of rats was higher than 16. 7 retool, and urine sugar presented as （＋＋＋）, indicating that diabetic rat model had been successfully established. After the successful establishment of rat model, rats were divided into normal group, model group, metformin, and metformin combined with simvastatin groups. BMD of the lower tibia was detected. Results BMD of the tibia of diabetic rats was lower than that in metformin group, while BMD in metformin group was also lower than that in metformin and simvastatin group, and BMD in normal group was the highest （P 〈 0. 01 ）. Conclusion BMD of the tibia under diabetic status is lower than that in normal state. BMD is improved significantly after the treatment, and the effect of metformin and simvastatin is superior to that of mctformin.