核桃GAI基因的克隆和序列分析

Cloning and Sequence Analysis of GAI Gene from Juglans regia L.

DELLA蛋白是赤霉素信号传导通路中一类重要的负调节因子。利用RT-PCR技术以香玲核桃叶片为试材,克隆得到核桃DELLA蛋白基因JrGAI。对基因和编码蛋白序列进行分析,结果表明,JrGAI基因开放阅读框(ORF)为1 837 bp,编码612个氨基酸;生物信息学分析表明,JrGAI编码蛋白具有DELLA蛋白的典型结构域;利用BLASTx和DNAMAN软件进行相似性分析发现JrGAI编码蛋白氨基酸序列与其它植物的DELLA蛋白具有较高的同源性,其中与辽宁2号核桃同源性最高,达到99%。核桃JrGAI基因的克隆为其进一步研究应用奠定了基础。

DELLA protein is an important negative regulator in gibberllins（GAs） signaling transduction pathway.DELLA protein gene,named JrGAI,was cloned from leaves of Juglans regia cultivar Xiangling using RT-PCR method.Then the sequences of JrGAI gene and deduced protein were analyzed.The results showed that the open reading frame（ORF） of JrGAI gene was 1 837 bp,encoding 612 amino acids.Bioinformatics analysis indicated that the typical domains of DELLA protein were found in the protein encoded by JrGAI gene.Similarity analysis by BLASTx and DNAMAN software showed that the deduced amino acid sequence of JrGAI gene was highly homologous with those of DELLA protein from other plants,and had the highest homology of 99% with Juglans regia cultivar Liaoning 2.The cloning of JrGAI gene laid foundations for its further research and application.