摘要
目的观察乌梅丸对2,4,6-三硝基苯磺酸(TNBS)诱导的溃疡性结肠炎大鼠脾脏组织β-arrestin1表达的影响。方法将56只SD大鼠随机分为空白对照组、模型组、美沙拉嗪组、乌梅丸组各14只,除空白对照组外,其余三组均应用TNBS灌肠;溃疡性结肠炎模型建成2 d后,空白对照组和模型组以蒸馏水、美沙拉嗪组以美沙拉嗪混悬液(50 g/L)、乌梅丸组以乌梅丸液(0.51 g/L)灌胃,均为每只3 mL,连续灌胃15 d后取脾脏组织,采用RT-PCR法检测β-arrestin1 mRNA,Western blot法检测β-arrestin1蛋白。结果与空白对照组比较,模型组β-arrestin1 mRNA、蛋白表达均升高(P均<0.05);与模型组比较,乌梅丸组、美沙拉嗪组β-arrestin1 mRNA、蛋白表达均下降(P均<0.05)。结论乌梅丸可下调TNBS诱导的溃疡性结肠炎大鼠脾脏组织β-arrestin1 mRNA、蛋白表达。
Objective To observe the effect of Wumeiwan on the expression of β-arrestinl in the spleen tissue of rats with 2, 4, 6-trinitrobenzenesulfonic acid （ TNBS）-induced ulcerative colitis. Methods Fifty-six SD rats were randomly divided into the control group, colitis model group, mesalazine group and Wumeiwan group （14 rats in each group）. Apart from the control group, rats in the other three groups were induced to experimental colitis by 2, 4, 6-trinitrobenzenesulfonic acid. And 2 days later, rats in the control group and colitis model group were administered intragastrically with normal sa- line at a dose of 3 mL, while rats in the mesalazine group and Wumeiwan group were intragastrically given mesalazine （50 g/L） and Wumeiwan （0.51 g/L） at a dose of 3 mL, respectively. All rats were treated for 15 d. Spleen tissue sam- ples were taken to detect the expression of mRNA of β-arrestinl by RT-PCR and the expression of β-arrestinl protein by Western blot. Results Compared with the control group, the expression of β-arrestinl mRNA and β-arrestinl protein was significantly increased in the colitis model group （ all P 〈 0.05） ; compared with the colitis model group, the expression of the mRNA and protein of β-arrestinl in the Wumeiwan and mesalazine groups was significantly decreased （ all P 〈 0.05 ）. Conclusion Wumeiwan can down-regulate the expression of the mRNA and protein of β-arrestinl inthe spleen tissue of rats with TNBS-induced ulcerative colitis.
出处
《山东医药》
CAS
2013年第27期23-25,共3页
Shandong Medical Journal
基金
国家自然科学基金资助项目(81072944)
湖北省自然科学基金资助项目(2011CDB386)
