GC-ECD法测定珠子参中有机氯农药残留量

GC-ECD Method for the Determination of Organochlorine Pesticides Residues in Rhizoma Panaois Majoris

目的:建立了气相色谱一电子捕获检测(ECD)法测定珠子参中有机氯农药残留方法。方法:采用AgilentDB-624毛细管柱(30m×0.32mm×1.8um);升温程序:初始温度100℃,15℃/min升至220℃,再以10℃/min升至250℃,保持17min;进样量:1.0ul。结果:六六六(BHC)、滴滴涕(DDT)、五氯硝基苯(PCNB)对照品均在0～400ug/l浓度范围内呈现良好的线性关系。结论:该方法准确可行,重复性好,可用于珠子参及其制剂的有机氯农残检测。

Objective: To establish a gas chromatography-electron capture detection(ECD) method for the determination of organochlorine pesticide residues in Rhizoma Panaois Majoris.Methods: Agilent DB-624 capillary column(30 m × 0.32 mm × 1.8 um) was used.Temperature program was to rise from 100℃ initial temperature to 220℃,15℃ / min,then to 250℃,10℃ / min,keeping for 17 min.Injection volume was 1.0ul.Results: The reference substance of BHC,DDT and PCNB were at 0 ～ 400 ug / L concentration range and showed a good linear relationship.Conclusion: The method is accurate and feasible with good repeatability and can be used to determine the organochlorine pesticide residues in Rhizoma Panaois Majoris and in its preparation.