电针对鱼藤酮诱导的帕金森病大鼠脑黑质氧化应激作用的影响

Effects of electric acupuncture on the oxidative stress in the rat of Parkinson’s disease abductioned by rotenone

目的:观察电针对鱼藤酮诱导的帕金森病大鼠模型氧化应激作用的影响。方法:所有实验动物随机分空白组、模型组、电针预处理组、电针治疗1周组、电针治疗2周组、电针治疗3周组、电针治疗4周组,每组10只,采用背部皮下注射鱼藤酮法制备模型。紫外分光光度法测定中脑黑质部位超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT)、丙二醛(MDA)含量的变化;同时采用免疫组化法观察黑质部位酪氨酸羟化酶(TH)阳性表达神经元。结果:与模型组相比,电针预处理组和电针治疗组均能提高GSH和SOD、CAT活力,降低MDA含量。大鼠黑质部TH阳性细胞明显增多。结论:电针能明显提高大鼠黑质区抗氧化应激能力。

Objective： To observe the effects of electric acupuncture on the oxidative stress in the rat of Parkinson＇s disease abductioned by rotenone. Methods： All rats were randomly divided into blank group, model group, electroacupuncture pretreatment group, electroacupuncture treat for one week group, electroacupuncture treat for two weeks group, electroacupuncture treat for three weeks group, electroacupunctnre treat for four weeks group. The Parkinson disease model was established by subcutaneous injection of rotenone. The contents of SOD, GSH, CAT, MDA were detected by ultraviolet spectrophotometry. And the expressions of tyrosine hydroxylase （TH） in substantia nigra were detected by immunohistochemistry. Result： Compared with the model group, the electroacupuncture pretreatment group and acupuncture group had the higher contents of SOD, GSH, CAT, and lower content of MDA. The TH positive dopamine neurons in substantia nigra was increased. Conclusion： The acupuncture can obviously increase the rat＇s resistance of oxidative stress.