高效液相色谱法测定盐炙补骨脂炮制前后三种主要成分的变化

Comparation of Three Constitutions between Psoralea corylifolia and Salt-processed One by HPLC

目的:建立同时测定补骨脂中补骨脂素、异补骨脂素和补骨脂酚3种主要有效成分含量的高效液相色谱方法,比较补骨脂盐炙品炮制前后三种成分含量的变化。方法:采用盐炙法对7个不同地区来源的补骨脂进行炮制,利用高效液相色谱法,甲醇-水梯度洗脱,检测波长246nm,测定炮制前后补骨脂素、异补骨脂素及补骨脂酚的含量。结果:盐炙法使补骨脂中的补骨脂素和异补骨脂素含量增加,使补骨脂酚的含量减少。结论:盐炙法使补骨脂中的三种主要有效成分含量发生了变化。

Objective：To establish a quantitative method of determination of psoralen,isopsoralen and bakuchiol in fruits of Psoralea carylifolia.and salt-processed one,and compare changes of the three kinds of component between them.Method：The content of psoralen,isopsoralen and bakuchiol in the salt-processed one from 7 different areas were determined by HPLC-gradient elution,determined on 246nm wavelength.Results：The content of psoralen and isopsoralen in salt-processed one were raising,and the content of bakuchiol was reducing.Conclusions：The method of salt process could change the content of psoralen,isopsoralen and bakuchiol in fruits of Psoralea carylifolia.