分光光度法测量生物组织中BPA含量

Determinate the BPA in Biological Samples by Spectrophotometry

建立了以3-甲氧基-甲亚胺-H为显色剂的二羟基硼酰苯丙氨酸(P-Boronophenylalanine,BPA)分光光度测量方法,并对该法在样品处理、试剂选择、条件测试等方面进行试验。研究BPA在正常小鼠体内的生物分布,以确定BPA的靶向性和有效性。

Boron neutron capture therapy （BNCT） is a new radiation therapy, of which clin- ical interest has focused primarily on the treatment of high-grade gliomas . At present, the most effective drug for BNCT is p-Boronophenylalanine（BPA） , becouse it has little side effect and low toxicity, persisted longer in tumors compared with related molecules. The method of 3-Methoxy-Methylenimine H spectrophotometry was established to measure the concentration of ^10B in biological samples. The biodistribution of the BPA was studied in normal mice.