大豆GmWRI1a基因克隆及生物信息学分析

Cloning and bioinformatics analysis of GmWRI1a in soybean

利用Phytozome数据库、NCBI网站等软件对大豆(Glycine max)基因组中WRI1同源基因进行生物信息学分析,发现大豆WRI1转录因子有2个拷贝,分布在第8和15号染色体上。以大豆未成熟种子cDNA为模板,克隆15号染色体WRI1转录因子的cDNA全长序列,命名为GmWRI1a。应用生物学软件发现,GmWRI1a蛋白含有2个AP2/EREBP结构域(60-225)。进化树分析表明,GmWRI1a包含在第一个分支里,与AtWRI1距离较近,推断其与AtWRI1属于同源基因,具有相似的功能。对与GmWRI1a蛋白存在相互作用的蛋白进行预测分析,发现与其相互作用的蛋白多数参与糖降解或质体脂肪酸合成,结果表明,GmWRI1a转录因子可能在控制种子发育中时从糖到油的碳流动中起重要作用。

Bioinformatics of WRl1 homologs in soybean genome was analyzed using Phytozome database and NCBI website. Two copies of soybean WRl1 homologs were found to distribute on chromosome 8 and 15, respectively. We cloned one gene on chrosome 15, and named GmWRl1a. GmWRl1a in soybean genome had two AP2/EREBP domains. Phylogenetic relations results showed that GmWRIla and AtWRl1 had the closest relationship. Therefore, preserved that GmWRl1a gene was a member of a plantspecific family of transcription factors （AP2/EREBP） that shared two copies of DNA-binding domain called the AP2 domain. Putative targets of GmWRlla encoded enzymes of late glycolysis, the fatty acid synthesis pathway. Thus, GmWRlla was putatively an important role in regulatory network controlling oil accumulation in seeds.