失水处理诱导番茄中JA相关基因表达的研究

Dehydration induced JA-related genes expression pattern in tomato

茉莉酸作为一种重要的植物激素,在调控植物对昆虫取食和腐生型病原菌等生物胁迫的抗性反应中具有重要作用。迄今为止,关于茉莉酸调控植物对非生物胁迫抗性的研究较少。以ABA和茉莉酸信号途径中一些番茄突变体为材料,利用荧光定量PCR方法研究失水处理对茉莉酸信号途径中相关标记基因的诱导情况,证明失水处理可诱导茉莉酸信号途径中相关标记基因的表达,这种诱导既依赖于ABA合成,又依赖于茉莉酸合成和信号传导,说明茉莉酸信号途径可能在调控番茄抗旱反应中发挥一定作用。

Jasmonates （JAs）, always known as important plant hormones, play pivotal roles in plant defense responses during insect attack and necrotrophic pathogen infection. To date, little is known about jasmonate-mediated regulation of plant responses to abiotic stresses. Using several ABA and JA mutants of tomato treated with dehydration stress, the induction patterns of some marker genes previously known to be involved in JA signaling pathway were analyzed through RT-PCR. The results showed that these marker genes could be significantly induced by dehydration treatment in an ABA and JA synthesis-dependent and also in a JA transduction-dependent manner. Therefore, this study lends credence to the possible involvement of JA pathway in tomato drought resistance.