摘要
Lipoic acid (LA) has received great attention in the area of gold surface functionalization. In this study, LA was employed as a linker for the attachment of antibody to the gold surface of surface plasmon resonance (SPR) chip. By using this chip in a homemade SPR immunosensor, low molecular weight compound 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) can be detected at a low level of 0.01 ng/mL. There is a good linear relationship(R2 =0.943 1) between the results of SPR biosensor and enzyme-linked immunosorbent assay(ELISA).
Lipoic acid （LA） has received great attention in the area of gold surface functionalization. In this study, LA was employed as a linker for the attachment of antibody to the gold surface of surface plasmon resonance （SPR） chip. By using this chip in a homemade SPR immunosensor, low molecular weight compound 2,3,7,8- tetrachlorodibenzo-p-dioxin （2,3,7,8-TCDD） can be detected at a low level of 0.01 ng/mL. There is a good linear relationship （R2 = 0.943 1） between the results of SPR biosensor and enzyme-linked immunosorbent assay （ELISA）.
基金
Supported by the National Natural Science Foundation of Tianjin (No. 09JCZDJC25700, 12JCZDJC29600 and KJXH2011-11)
