摘要
基于阳离子表面活性剂十六烷基三甲基溴化铵(CTAB)对蒽环类抗生素阿霉素的荧光强度有增敏作用,通过考察CTAB浓度、缓冲液pH及浓度、有机溶剂等对阿霉素荧光强度的影响,建立胶束增敏荧光光度法测定阿霉素的新方法。在体系为17mmol/L的硼酸缓冲液(pH 6.0)且含60%(v/v)乙醇、1.9mmol/L CTAB的条件下,阿霉素在0.5～120 ng/mL浓度范围内荧光强度呈良好的线性(r=0.999 2),检出限(3σ)为0.161 ng/mL。该方法用于测定兔血样中阿霉素时,回收率在93.7%～105.6%之间,相对标准偏差小于1.5%。结果表明该方法具有较好的准确度与灵敏度,可用于临床监测。
Doxorubicin is a kind of anthracycline antibiotics and the fluorescence of it can get enhanced in the presence of hexadecyl trimethyl ammonium bromide （CTAB） in ethanol-borate buffer solution. A new fluorescent spectrophotometry based on this principle has been developed to determine doxorubicin in rabbit serum. Method development included study of the effects of buffer pH, buffer concentration, organic solvent and CTAB concentration on the fluorescence intensity of DOX. The optimum conditions were pH 6.0 17 mmol/L borate buffer, 60% v/v ethanol and 1.9 mmol/L CTAB. The fluorescence intensity was linearly related to the concentration of doxorubicin in the range of 0.5 to 120 ng/mL with a correlation coefficient of 0.999 2 under the optimum conditions. The detection limit （3σ） is 0.161 ng/mL. The proposed method was satisfactorily applied to the determination of doxorubicin in rabbit serum. The recovery was within the range of 93.7%-105.6% （ RSD 〈l .5% ）. The results showed that this method had grcat accuracy and sensitivity, which is widely applied in chinical test.
出处
《中国测试》
CAS
北大核心
2013年第2期44-47,共4页
China Measurement & Test
基金
国家自然科学基金项目(21277109)
四川省高校科技创新团队建设项目(2010008)
