山东省小麦禾谷孢囊线虫的分布及其rDNA-ITS分析

Distribution and rDNA-ITS analysis of cereal cyst nematode in Shandong Province

采用随机抽样的方法,对中国山东省7个地区19个乡(镇)的小麦孢囊线虫的分布和发生情况进行调查,并根据形态和rDNA-ITS分子特征对病原线虫种类进行鉴定。结果表明:在山东省临沂、莱芜、淄博、潍坊、东营、威海、烟台等7个地区发生的孢囊线虫均为禾谷孢囊线虫(Heterodera avenae Wollenweber),其检出率为70.9%,且以东营市和潍坊市的孢囊数及卵量最高,烟台市的孢囊数及卵量最低。

The cereal cyst nematode（Heterodera avenae Wollenweber） is the most threatening nematode on wheat and cereal crops in China.The occurrence and distribution of Heterodera spp.were investigated by random sampling method from 19 town or counties of Shandong Province.The species was identified as Heterodera avenae with the morphological and rDNA-ITS analysis.The cereal cyst nematode was detected from 70.9 percent samples collected from Linyi City,Laiwu City,Zibo City,Weifang City,Dongying City,Weihai City and Yantai City.The highest cyst and egg number appeared in Dongying City and Weifang City,the lowest cyst and egg number appeared in Yantai City.