等长收缩法测定小鼠阻力血管舒缩反应

Isometric Force Measurement of Vasomotor Reaction in Mouse Resistance Arteries

目的:通过等长收缩法检测小鼠肠系膜动脉的舒缩效应,为研究小鼠阻力血管张力变化及其调控机制提供直观、可靠的实验方法。方法:取C57BL/6成年雄鼠肠系膜动脉制备1.0mm长血管环,在苯肾上腺素预收缩的基础上,观察乙酰胆碱、花生四烯酸对小鼠肠系膜动脉张力变化的影响。结果:未加入一氧化氮合酶抑制剂,乙酰胆碱能很好地诱导小鼠肠系膜动脉血管环的血管舒张效应,该效应随浓度的增加而增大;而在加入一氧化氮合酶抑制剂的情况下,花生四烯酸亦能很好地诱导小鼠肠系膜动脉血管环浓度依赖性的血管舒张效应。结论:成功建立用等长收缩法测定以肠系膜动脉为代表的小鼠阻力血管舒缩反应的方法。

Objective： To detect vasomotor reaction of mouse mesenteric arteries with isometric force measurement in order to establish a direct and reliable method to study the regulatory mechanisms of vessel function in mouse resistance arteries.Me^od： Vascular rings （ 1.0 mm ） were prepared using the mesenteric artery of C57BL/6 adult male mice to observe the effects of acetylcholine and araehidonie acid on vasomotor reaction of vessels pre- contracted by phenylephrine.Result： Aeetylcholine induced dose-dependent vasodilation of mesenteric arterial tings in the absence of nitric oxide synthase inhibitor.Arachidonie acid also evoked vasodilation mesenterie artery in the presence of nitric oxide synthase inhibitor.Conclusion： A method of isometric force measu^ment is successfully established to detect vasomotor reaction in mouse resistance vessels such as mesenteric artery.