摘要
为探讨饲料中不同大豆卵磷脂/鱼油比例对斜带石斑鱼稚鱼成活率、生长行为、饲料利用以及肠道组织学的影响,本实验设计了4种添加不同大豆卵磷脂/鱼油比率的微颗粒饲料:0%/22%(饲料1),5%/14%(饲料2),10%/8%(饲料3)以及15%/7%(饲料4),饲喂斜带石斑鱼稚鱼。每种实验饲料均设3个平行。试验持续40d。结果表明,饲料中不同大豆卵磷脂/鱼油比例对斜带石斑鱼稚鱼生长以及成活率有显著影响(P≤0.05)。饲料3和饲料4的增重分别为:(449.6±11.4)g和(588.3±61.7)g,显著高于饲料1的数值(185.0±2.5)(P≤0.05)。饲料1鱼体脂肪含量为:(154.7±12.8)g·kg-1,显著低于其他饲料组(P≤0.05),但其鱼体蛋白含量为(728.3±25.5)g·kg-1,显著高于其他饲料组。饲料效率、肥满度、特定生长率、摄食量和蛋白质效率以及终末体重中,4种饲料之间无显著性差异。随饲料卵磷脂/鱼油比例升高,石斑鱼稚鱼肠道脂滴生成数量出现下降,且在饲料4中未观察到肠道脂滴。研究表明斜带石斑鱼稚鱼生长发育阶段需要较高的饲料卵磷脂/鱼油比例(如:15%/7%)。
Four formulated microdiets supplemented with different soybean lecithin/fish oil (SL/FO) ratios: Diet1(0%/22% ) ,Diet2(5%/14% ) ,Diet 3 (10%/8%) and Diet 4 (15%/7% ) were fed to 40-day-old juvenile grouper Epinephelus coioides after hatching. Each experimental diet was distributed in triplicate tanks. The survival, feed efficiency,growth performance and intestine histology of the grouper juveniles were monitored after a 40-day growth trial. Results showed that different dietary SL/FO ratios significantly affected the growth and survival ( P≤ 0. 05) of the grouper juveniles. The juveniles fed Diet 3 (10% /8% ,SL/FO) and Diet 4 (15/7,SL/FO) gave significantly higher weight gain ( WG) than those fed Diet 1 ( 0% /22% ,SL/FO) . The juveniles fed Diet1 (0%/22% ,SL/FO) had obviously lower whole body lipid content than those fed other diets ( P≤0. 05) ,but had significantly higher whole body protein content than those fed other diets ( P 0. 05) . For all treatments no significant differences were observed in feed efficiency (FE) ,hepatosomatic index ( HSI) ,viscerosomatic index ( VSI) and condition factor ( CF) ,specific growth rate ( SGR) ,feed intake (FI) ,protein efficiency ratio (PER) ,and final body weight (FBW) . Increase in dietary soybean lecithin/fish oil ( SL/FO) ratio reduced lipid droplets in intestine enterocytes of grouper juvenile. It is concluded that the grouper juvenile microdiets should have a high dietary soybean lecithin/fish oil ratio (15%/7% ) .
出处
《热带生物学报》
2013年第2期111-118,共8页
Journal of Tropical Biology
基金
supported by Key Projects in the National Key Science & Technology Program during the Twelfth Five-Year Plan Period(2012AA10A414,2012AA10A407)
the project of Start-up Fund of Hainan University(kyqd1041)
Youth Foundation of Hainan University(qnjj1009)
Foundation of Hainan Educational Committee(Hjkj2011-15)
partially supported by Key Laboratory for Sustainable Utilization of Marine Bio-resources,Chinese Academy of Sciences(LMB101008)~~
关键词
斜带石斑鱼稚鱼
大豆卵磷脂
鱼油
生长
成活率
Epinephelus coioides juvenile
soybean lecithin
fish oil
growth
survival