黄姜花组培快繁技术

Rapid Propagation of Hedychium flavum Roxb.

以黄姜花的笋芽为外植体,研究了外植体的消毒、丛生芽的增殖及生根培养等关键步骤的配方。结果表明:1)利用ρ=1g·L-1的升汞对外植体进行消毒的最佳时间为12min;2)黄姜花丛生芽增殖的较优配方为:3/4MS+6-BA3.5mg·L-1+水解酪蛋白1000mg·L-1+蔗糖30g·L-1,继代周期25d,增殖倍数为5.83;3)1/2MS+NAA0.5mg·L-1的生根培养基效果最好,平均生根数达9.17条,平均根长2.73㎝,植株健壮;4)将试管苗移裁在V河砂︰V表土=1︰1的基质中,25d后成活率可达91.67%,植株生长良好。

Shoots newly sprouting from underground rhizome of Hedychium flavum Roxb. ere used as explants for rapid propagation. The explants were sterilized,induced into clustered shoots,generated into plantlets and rooted on different media,and then transferred onto the mixed substrates of sand and surface soil for culture. The explants sterilized with HgCl 2 （ 1 g·L-1 ） for 12 min gave best result. The optimal medium for proliferation of clustered shoots was 3/4MS＋6-BA3.5 mg·L-1＋CH 1 000 mg·L-1＋sugar 30 g·L-1 . On this medium clustered shoots were subcultured for 25 days as a cycle at a proliferation coefficient of 5. 83. The plantlets were best rooted on the rooting medium of 1/2MS ＋ NAA 0. 5 mg·L^–1 ,producing upto 9. 17 roots/plantlet with an average root length of 2. 73 cm,and the rooted plantlets grew robust and 5. 43 cm tall by average. The rooted plantlets transferred onto the substrate of river sand 50% ＋ surface soil 50% （ V ︰ V） for culture gave 91. 67% survival after 25 days of transfer and grew well.