脑微血管内皮细胞TNFR差异表达对rhTNF开放血肿瘤屏障的影响

Effect of TNFR differentially expressed on brain microvascular endothelial cells for rhTNF open the blood-tumor barrier

目的观察脑微血管内皮细胞的肿瘤坏死因子受体(TNFR)差异表达对rhTNF开放血肿瘤屏障的影响。方法建立TNFR1/TNFR2高表达的脑微血管内皮细胞株,并与C6胶质瘤细胞一起通过Transwell小室构建体外血肿瘤屏障模型。免疫荧光技术检测脑微血管内皮细胞TNFR的表达水平。荧光分析法测定给予rhTNF后的血肿瘤屏障通透性变化。结果脑微血管内皮细胞的TNFR1高表达时,rhTNF开放血肿瘤屏障的程度最大。而TNFR2高表达时,血肿瘤屏障开放程度与对照组相比无明显差异。结论 rhTNF可能是通过与脑微血管内皮细胞的TNFR1结合而引起血肿瘤屏障开放的。

Objective To observe effect of TNFR differentially expressed on brain microvascular endothelial cells for rhTNF open the blood-tumor barrier.Methods Brain microvascular endothelial cell（BMEC） of TNFR1/TNFR2 highly expression line was established and blood tumor barrier in vitro model with C6 glioma cells through Transwell was built.Using immunofluorescence to detect TNFR expression levels in brain microvascular endothelial cells.Fluorescence analysis method was used to determine blood tumor barrier permeability changes after given the rhTNF.Results Blood-tumor barrier openess reached maximum by rhTNF when TNFR1 high expression on brain microvascular endothelial cells,but high expression of TNFR2 did not cause significant blood-tumor barrier openness than control group.Conclusion rhTNF may combine TNFR1 on brain microvascular endothelial cells and open the blood tumor barrier.