松龄血脉康预处理对脑缺血再灌注大鼠全脑及血清IL-6表达的影响

Effect of Songling Xuemaikang pretreatment on the expression of interleukin-6 after focal cerebral ischemia-reperfusion in rats

目的探讨松龄血脉康预处理对脑缺血再灌注大鼠全脑及血清白细胞介素-6(Interleukin-6,IL-6)表达的影响。方法 60只雄性SD大鼠,随机分为松龄血脉康(SL-xmk)预处理组、假手术组、对照组。SL-xmk预处理组采用SL-xmk悬浮液(937.5mg/kg)对大鼠进行为期8w的预防性灌胃处理(n=20),假手术组(n=20)、生理盐水对照组(n=20)采用等容量生理盐水预防性灌胃处理。在预处理时程终点采用线栓法制作大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)2h再灌注24h模型。观察SL-xmk对预处理MCAO大鼠神经功能缺损评分、脑水含量和脑梗死体积的影响。采用ELISA法检测全脑及血清IL-6含量。结果 SL-xmk预处理后全脑和血清IL-6表达显著下降,缺血脑组织含水量和脑梗死体积也明显降低。结论松龄血脉康预处理能显著抑制脑缺血再灌注大鼠脑组织和血清IL-6的表达,减轻神经功能缺损,降低脑组织水含量,缩小梗死体积。

Objective To Study the effect of Songling Xuemaikang（SL-xmk） preconditioning on the expression of interleukin-6（IL-6） after cerebral ischemia-reperfusion injury in rats.Methods 60 adult male Sprague-Dawley rats were randomly allocated into SL-xmk pretreatment（n = 20）,sham operation（n = 20）,and normal saline control group（n = 20）.Sham and normal saline control groups were both treated with 1ml/100g isotonic saline,and the pretreatment group of 8w were treated with 937.50mg/kg of SL-xmk 2h before receiving MCAO followed by 24h reperfusion.The neurologic impairment scores,brain water content and infarct area in right hemisphere were observed after ischemia for 2h followed by 24h reperfusion,ELISA method was used to determine IL-6 content in whole brain tissue and blood serum.Results SL-XMK in whole brain and serum IL-6 expression after pretreatment decreased significantly,the brain water content and cerebral infarction volume were also decreased.Conclusion SL-xmk pretreatment may significantly inhibiti the expression of IL-6 in whole brain tissue and blood serum in focal cerebral ischemia-reperfusion rats and reduce brain water content and infarct volume.