16种玉兰亚属植物的AFLP分析

AFLP Analysis for 16 Species of Subgenus Yulania

对16种玉兰亚属植物进行了AFLP分析,共获得1 728个AFLP条带,其中具有多态性的条带为908条,多态率达52.5%。根据AFLP聚类结果对16种玉兰亚属植物的亲缘关系及新种的确定进行了探讨,研究结果支持将天目玉兰(Magnolia.amoena)和望春玉兰归为同一组;厚叶玉兰(M.crassifolius)和红花玉兰(M.wufengen-sis)亲缘关系最近,结合形态学特征,推测厚叶玉兰可能是红花玉兰的多瓣化变种之一;倾向于将景宁玉兰(M.si-nostellata)单独作为种的分类处理,并将景宁玉兰、天目玉兰与望春玉兰同归为望春玉兰组;研究中,伏牛山玉兰(M.funiushanensis)独特的聚类关系与已有的分类系统均不同,其确切的分类学地位还需更多的研究来验证;AFLP分子标记的研究结果还显示出以萼片状花被片、花被片颜色作为玉兰亚属分组依据存在欠缺和不足。

An experiment was conducted to analyze AFLP for 16 species of Subgenus Yulania. We obtained a total of 1728 AFLP bands, and 908 of them were polymorphic bands and the polymorphic rate was 52.5%. By the clustering result, we ob- tained a fine phylogenetic relationship for 16 species of Subgenus Yulania and made a further discussion about the identifi- cation of new species. The phylogenetic relationship showed that M. amoena and M. biondii might be in the same group. M. crassifolius and M. wufengensis have the closest relationship among those species according to the clustering result. Combined with their morphological characteristics, we approved that M. crassifolius might be a multi-petal variety of M. wufengensis. We agreed with that M. sinostellata should be treated as a single species in classification and be in the same group, section Buergeria （spaeh） Dandy with M. amoena and M. biondii. M. funiushanensis has a unique clustering posi- tion in our study, is different from all the current classification systems. Therefore, further classification works are necessa- ry to discover its scientific polymorphic position. The result of AFLP shows that some morphological characteristics, such as seual-like petal and petals color, are defective and not enoucJa for the classification of subgenus Yulania.