水杨酸诱导重瓣玫瑰对白粉病的抗性

Salicylic Acid Induced Resistance of Rosa rugosa ‘Plena’ to Sphaerotheca pannosa (Wallr.)

喷施不同浓度的水杨酸(SA),研究其引发重瓣玫瑰对白粉病的诱导抗性,实验结果表明,不同浓度SA诱导处理均对重瓣玫瑰白粉病有抑制效果,SA处理显著减低了重瓣玫瑰叶片白粉病病情指数,0.5 mmol/L SA诱导效果最好。不同浓度SA诱导(接菌)后,对重瓣玫瑰叶片苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD)活性有显著影响,且对总酚质量分数、木质素相对值的影响明显。经0.5 mmol/L SA诱导后,叶片PPO活性、总酚质量分数均在诱导(接菌)后第7(5)天时达到最大值,分别是CK1(喷施清水)的1.55、1.71倍,是CK2(喷施清水+接种白粉菌)的1.33、1.50倍,且与CK1、CK2相比差异极显著(P<0.01)。1.0 mmol/L SA诱导(接菌)后PAL、POD活性在第7(5)天时达到最大值,分别是CK1的1.68、2.06倍,是CK2的1.35、1.56倍,且与CK1、CK2相比差异极显著(P<0.01)。0.5 mmol/L SA处理在整个试验期间诱导的木质素相对值均显著高于CK1、CK2。

By sparying different concentration of salicylic acid （SA） on Rosa rugosa Plena , we investigated their influence on the changes of several defense enzymes activity as well as secondary metabolite in leaves of R. rugosa Plena. The effec- tiveness of different content SA on the resistance of Sphaerotheca pannosa （ Wallr. ） is obvious. SA spraying can signifi- cantly decrease disease indexes of S. pannosa （ Wallr. ） in R. rugosa ＇ Plena＇ leaves especially by 0.5 mmol/L SA spra- ying. The activities of three kinds of protective enzymes, PAL, PPO and POD, the content of total phenols and the relative value of lignin are obviously induced by SA. After induced with 0.5 mmol/L SA, the PPO activities and the content of to- tal phenols reaches their maximum value on the seventh （fifth） day after inducing （ inoculating of S. pannosa （ Wallr. ） ）, and their activities are 1.55 times （P〈O. 01 ） and 1.71 times （P〈0.01） higher than that in control No. 1 which is sprayed with pure water only （ CK1 ）, and were 1.33 times （P〈0.01） and 1.50 times （P〈O. 01 ） higher than that in the control No. 2 （CK2） which is sprayed with pure water then inoculated with S. pannosa （Wallr.） spores, and their those differ- ences are very significant. After induced with 1.0 mmol/L SA, the PAL and POD activities reach their maximum on the seventh （fifth） day after inducing （ inoculating of S. pannosa （ Wallr. ） ）, and their activities are 1.68 times （P〈0.01） and 2.06 times （P〈O. O1 ） higher than that of CK1, 1.35 times （P〈0.01） and 1.56 times （P〈0.01） higher than that of CK2, and those differences are obvious in significant level. After induced with O. 5 mmol/L SA, the relative value of lignin is significantly higher than that of CK1 and CK2 during the whole test.