复方白头翁胶体制剂的研制及体外抗阴道毛滴虫耐药株的实验研究

Preparation of colloid solution of compound radix pulsatillae extract and its in vitro pharmacodynamics effect on resistant trichomonas vaginalis

目的:探讨复方白头翁胶体制剂对阴道毛滴虫耐药株的抑制作用,开发出适合女性泌尿生殖道阴道毛滴虫感染的新型药物释放系统。方法:白头翁提取物与蚕蛹复合氨基酸及铜绿等矿物质络合成胶体溶液制剂,以肉汤稀释法测定不同浓度的制剂对阴道毛滴虫耐药株的灭活效应。结果:复方白头翁提取物胶体制剂含量为1.0 g/ml、0.1 g/ml时,两组的滴虫死亡率均高于空白对照组(P<0.01),滴虫的死亡率随着药物浓度的增加而增加,同一药物浓度随着作用时间的延长滴虫死亡率也增高。药物作用24 h后,滴虫全部死亡。结论:复方白头翁胶体制剂可明显抑制阴道毛滴虫耐药株,具有高活性抗阴道毛滴虫耐药株生长的药效学作用。

Objective： To develop the new kind of drug - releasing system containing colloid solution of compound Radix Pulsatil- lae extract （CRPE） on trichomonas vaginalis and explore the inhibitory molecular mechanism of anti-trichomonas vaginalis. Methods： The colloid solution agent containing total alkaloids of Radix Pulsatillae, bombyxmoril＇s compound amino acids and minerals including Cu2 （OH） and borax were prepared, the death rate was determined on resistant strain with broth dilution assay. Results ： The colloid solution of 1.0 g/ ml and 0. 1 g/ml on resistant Trichomonas Vaginalis were higher than negative control respectively. Compared with the negative groups, there was statistic meaning between the two groups （P 〈0.01 ） . Conclusion： The colloid solution had a significant inhibitory effects in vitro on resistant trichomonas vaginalis.