摘要
用农杆菌介导法将高亲和性钾离子转运体基因(HAK)和Bar基因转入5个优良玉米自交系7922、P138、265、238和271中,并对影响其遗传转化效率因素进行了优化.经PCR和RT-PCR检测证实获得阳性植株.除草剂涂抹实验证明Bar基因已经整合进玉米基因组.用不同浓度的盐溶液处理转基因植株和对照植株,发现转基因植株叶片中的K+含量、脯氨酸和叶绿素含量均高于未转化植株,而Na+含量低于未转化植株,表明HAK基因已经整合进玉米基因组,并通过过量表达提高了植株的耐盐性.
The HAK and Bar genes were transferred into five different inbred lines of maize, including 7922, P138, 265, 238 and 271, by Agrobacterium-mediated transformation. Factors influencing transformation efficiency were optimized. Transgenic plants, which are resistant to phosphinothricin （PPT）, were acquired by PCR and RT-PCR. When treated with different concentration of NaC1, higer K＋, proline and chlorophyll contents were observed in transgenic leaves than in wild type leaves, but Na＋ content in transgenic leaves was obviously lower than in wild type leaves. These results show that tIAK gene is integrated into maize genome and transcribed normally. Over-expressing of HAK gene enhances the salt tolerance of transgenic maize.
出处
《天津大学学报（自然科学与工程技术版）》
EI
CAS
CSCD
北大核心
2013年第7期659-665,共7页
Journal of Tianjin University：Science and Technology
基金
国家转基因生命新品种培育重大专项资助项目(2009ZX08003-019B)
国家转基因生物新品种培育重大专项资助项目(2011ZX08003-005)
国家自然科学基金资助项目(31271793
31271419)
教育部高等学校博士学科点专项科研基金资助项目(20100032110060)
关键词
玉米自交系
农杆菌介导
HAK
耐盐性
maize inbred line
Agrobacterium-mediated
HAK
salt tolerance
