蛋氨酸和裂壶藻强化对卤虫体内游离氨基酸含量的影响

Effects of enrichment on the free amino acid content in Artemia nauplii using methionine or Schizochytrium

以卤虫(Artemia parthenogenetica)无节幼体作为试验材料,研究蛋氨酸或裂壶藻(Schizochytriumsp.)强化对卤虫体内游离氨基酸(FAA)含量的影响。试验分为4组,分别为蛋氨酸(Met)组、裂壶藻组、蛋氨酸加裂壶藻组以及对照组,试验进行16 h,每4 h取样1次。结果显示:试验结束时,蛋氨酸组的卤虫体内游离Met含量显著高于裂壶藻组(P<0.05)。除对照组的丝氨酸(Ser)、谷氨酸(Glu)、甘氨酸(Gly)含量显著高于蛋氨酸+裂壶藻组外(P<0.05),其他所有的必需氨基酸及非必需氨基酸含量在16h时都没有显著差异。试验过程中FAA水平随时间的变化趋势为:对照组和蛋氨酸组基本上呈现先减少再升高的趋势,且16 h和0 h的含量差别不大;裂壶藻组和蛋氨酸加裂壶藻组的卤虫,其体内FAA含量基本上呈下降趋势,或小幅升高后下降,16 h时的含量大多低于0 h时。试验结果表明:给卤虫无节幼体强化Met可以提高其体内Met含量,对其他种类的FAA影响不大。

Effects of different enrichment materials on the free amino acid content in Artemia nauplii were studied. The experiment was carried with 4 groups, including methionine group, Schizochytrium group, methionine plus Schizochytrium group and control group. The experiment lasted 16 hours, and was sampled every 4 hours. The re- sults showed that the content of free methionine of Artemia nauplii in methionine group was significantly higher than that in Schizochytrium group （P〈0.05）. No significant differences were observed for all essential amino acids and nonessential amino acids except that Ser, Glu and Gly content in control group was significantly higher than those in methionine plus Schizochytrium group at the end of the experiment （P〈0.05）. The variation trends of the FAA level in the control group was similar to the methionine group： the content of FAA declined at first and then increased, and the content was similar between 0 and 16h. But in methionine plus Schizochytrium group and Schizochytrium group, the content of these FAA in Artemia nauplii mainly declined during the experiment or slightly increased first, and then declined, and the content of most FAA at 16 h was lower than that at 0 h. The re- suits point that enriching Artemia nauplii with methionine is feasible and could increase the content of methionine but there is little influence on FAA profile.