聚乳酸/茶多酚复合纳米纤维膜的抗菌机制及性能

Antibacterial mechanism and performance of PLA/TP composed nanofiber membrane

将茶多酚(TP)添加到聚乳酸(PLA)纺丝溶液中,以静电纺丝法制备PLA/TP复合纳米纤维膜。通过红外光谱(FT-IR)测试、抑菌圈法、振荡烧瓶法及透射电镜(TEM)对复合纳米纤维膜的成分、抗菌性能及抗菌机理进行研究。FT-IR测试结果验证了PLA/TP复合纳米纤维膜中通过价键的结合使二者复合在一起。抗菌测试结果显示:随着TP含量增加,复合纳米膜抗菌性能提高。对大肠杆菌和金黄色葡萄球菌的抑菌圈宽度分别从3.67和3.71 cm增加到5.17和5.67 cm,抑菌率从20.6%和21.3%提高到96.9%和97.6%。TEM观察结果表明,PLA/TP复合纳米纤维膜能够破坏菌体细胞膜的完整性,最终导致菌体细胞的死亡。

Polylactic acid （PLA） solution mixed with tea polyphenol （TP） was electrospun into PLA/TP composed nanofiber membrane whose composition, antibacterial performance and mechanism were investigated using infrared spectroscopy （FT-IR） , inhibition zone method, oscillation flask method and transmission electron microscope （TEM）. FT-IR test showed that the TP and PLA combined well by valence bonds on PLA/TP nanofiber membrane. Antibacterial experiment indicated that the antibacterial performance of the membrane increased as TP content increased. The widths of inhibition zone against Escherichia coli and Staphylococcus aureus increased from 3. 67 and 3. 71 cm to 5. 17 and 5. 67cm respectively. The inhibitory rate increased from 20.6% and 21.3% to 96.9% and 97.6% respectively. TEM examination indicated that PLA/TP composed nanofiber membrane can destroy the integrity of cell membrane of bacteria and finally lead to the death of bacteria.