荧光定量聚合酶链反应分型法检测乙型肝炎病毒B、C基因型的研究

Research of Quantitative PCR Genotyping Detecting B,C Genotype of the Hepatitis B Virus

目的探讨用荧光定量聚合酶链反应(PCR)分型法检测乙型肝炎病毒(HBV)B、C基因型,了解不同类型HBV感染者中HBV基因型的分布特点。方法选取2010年9月至2012年10月在成都市第五人民医院就治的乙型肝炎患者135例作为研究对象,用荧光定量PCR分型法检测135例HBV感染者的HBV基因型。结果荧光定量PCR分型法成功分型131例(97.04%)样本,4例(2.96%)未检出,其中,B/C混合型28例(20.74%);C型17例(12.60%);B型86例(63.70%)。B基因型的病毒载量显著高于C基因型、B/C混合型(P<0.05)。B基因型在慢性乙型肝炎、慢性重型乙型肝炎和急性乙型肝炎患者中所占百分比显著高于C基因型与B/C混合型(P<0.05)。结论本地区的HBV基因型以B基因型为主,其次是C基因型、B/C混合型。

Objective To explore B,C genotype of hepatitis B virus by quantitative PCR typing method and understand the distribution characteristics of different types of hepatitis B virus infection by HBV genotype.Methods From September 2010 to October 2012135 cases of HBV infection treated in the Fifth People＇s Hospital of Chengdu City were selected as object of study,which were detected by quantitative PCR typing method.Results Quantitative PCR typing method successfully genotyped 131 cases（97.04%） of the sample,and 4 cases（2.96%） were not detected.28 cases（20.74%） were B / C hybrid.17 cases（12.60%） were C genotype.86 cases（63.70%） were B genotype.The difference was statistically significant（P 0.05）.The genotype B percentage in chronic hepatitis B,severe chronic hepatitis B and acute hepatitis B patients and the was higher than that of mixed C and B / C,the difference was statistically significant.Conclusion The major HBV infection in the area is B genotype,followed by C genotype and B / C hybrid.