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水稻白叶枯病抗性基因Xa31(t)候选基因表达载体的构建

Construction of Expression Vector for Candidate Gene of Bacterial Blight Resistance Gene Xa31(t) in Rice
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摘要 [目的]构建水稻白叶枯病抗性基因Xa31(t)候选基因的表达载体。[方法]利用长片段PCR在抗白叶枯病水稻品种扎昌龙中扩增长10.6 kb的候选基因,将其通过Asc I单酶切克隆至植物表达载体pCAMBIA1300中,并对阳性克隆进行质粒PCR、酶切检测和测序分析。[结果]试验成功构建了水稻白叶枯病抗性基因Xa31(t)候选基因的表达载体。[结论]该研究为进一步研究Xa31(t)基因的功能奠定了基础。 [Objective] The paper was to construct the expression vector for candidate gene of the bacterial blight resistance gene Xa31(t) in rice.[Method] A 10.6 kb-length candidate gene was amplified form the genomic DNA of bacterial blight resistance rice cultivar ’Za Chang Long’(ZCL) by long-range PCR,and the gene was cloned into plant expression vector pCAMBIA1300 by Asc I digestion.The positive clones were detected by plasmid PCR,restriction enzyme digestion and sequencing.[Result] The expression vector for candidate gene of the bacterial blight resistance gene Xa31(t) in rice was constructed successfully.[Conclusion] The research laid a foundation for the further study of the function of Xa31(t) gene.
出处 《安徽农业科学》 CAS 2013年第15期6611-6612,6629,共3页 Journal of Anhui Agricultural Sciences
基金 教育部科学技术研究重点项目(210265)
关键词 水稻 白叶枯病 候选基因 长片段PCR 表达载体 Rice Bacterial blight Candidate gene Long-range PCR Expression vector
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