血红铆钉菇子实体对人肝星状细胞LX-2和人肝癌细胞Hep G2增殖的影响

Effects of Chemical Fractions from Chroogomphus rutilus Fruiting Body on the Proliferation of Human Hepatic Stellate Cells LX-2 and Human Liver Carcinoma Hep G2 Cells

[目的]探讨血红铆钉菇子实体对人肝癌细胞HepG2和人肝星状细胞LX-2增殖的影响,筛选出血红铆钉菇子实体抗肝纤维化和抗肿瘤作用的活性部位。[方法]利用天然药物化学方法提取得到血红铆钉菇子实体的石油醚、二氯甲烷、乙酸乙酯、正丁醇、水和粗多糖提取物,应用MTT法观察血红铆钉菇子实体不同溶剂提取物对体外培养的人肝星状细胞LX-2和人肝癌细胞Hep G2增殖的抑制作用,通过倒置显微镜观察细胞的形态学变化。[结果]血红铆钉菇子实体石油醚、二氯甲烷和乙酸乙酯提取物均对人肝星状细胞LX-2和人肝癌细胞Hep G2具有抑制增殖作用,显微镜观察结果表明,均可改变LX-2细胞和Hep G2细胞的形态。[结论]石油醚、二氯甲烷和乙酸乙酯提取物是血红铆钉菇子实体抗肝纤维化和抗肿瘤作用的主要活性部位。

[Objective] To study the effects of chemical fractions from Chroogomphus rutilus fruiting body on the proliferation of human hepatic stellate cells LX-2 and human liver carcinoma Hep G2 cells,and to screen the active fractions with anti-hepatic fibrosis and anti-tumor activity from C.rutilus fruiting body.[Method] The polysaccharide,petroleum ether,dichloromethane,ethyl acetate,n-butanol and aqueous fractions from C.rutilus fruiting body were obtained by the methods of nature products chemistry.MTT assay was used to determine the effects of above fractions on the proliferation of human hepatic stellate cells LX-2 and human liver carcinoma Hep G2 cells.Morphological changes were observed through invert microscope.[Result] The petroleum ether fraction,dichloromethane fraction and ethyl acetate fraction showed inhibitory activities on human hepatic stellate cells LX-2 with IC50 values at 519.6,526,675.7 μg/ml,respectively,and human liver carcinoma Hep G2 cells with IC50 values at 727.6,610.1,618.1 μg/ml,respectively.Morphological change of apoptosis body formation was observed by invert microscope.[Conclusion] Among polysaccharide and solvent fractions from C.rutilus fruiting body,the petroleum ether fraction,dichloromethane fraction and ethyl acetate fraction have anti-hepatic fibrosis and anti-tumor activities as the main active parts.