摘要
[目的]建立香水百合花中黄酮含量的测定方法。[方法]以香水百合花为材料,通过超声波提取样品中的黄酮类物质,采用光度分析法对香水百合花中总黄酮含量进行测定。[结果]试验得出,该方法的回归方程为:A=0.012 2c-0.000 6,r2=0.999 2,线性范围是4.1～24.6μg/ml,加标回收率是99.5%～103.1%,SD=0.016%,RSD=2.9%。[结论]该方法简便、准确、快速,稳定性符合要求,可用于测定香水百合花总黄酮的含量。
[Objective] To establish the method for determination of flavonoids in perfume lily.[Method] With perfume lily as material,flavonoids were extracted by ultrasonic power.And spectrophotometric method was used to determine total flavonoid content in perfume lily.[Result] The regression equation is A=0.012 2c-0.000 6,r2=0.999 2,linear range 4.1-24.6 μg/ml,The standard addition recovery rate is 99.5%-103.1%,SD=0.016%,RSD=2.9%.[Conclusion] The method is accurate,simple,stable,which could be used to determine total flavonoid content in perfume lily.
出处
《安徽农业科学》
CAS
2013年第15期6900-6901,共2页
Journal of Anhui Agricultural Sciences
关键词
香水百合花
总黄酮
光度分析法
Perfume lily
Total flavonoid
Spectrophotometric method
