糯玉米基因组DNA提取方法的比较

Comparison on Three Methods of Extracting Waxy Maize Genomic DNA

以糯玉米的胚为材料,采用SDS法、CTAB法和碱煮法提取糯玉米基因组DNA,样品经紫外分光光谱吸收、琼脂糖电泳、PCR扩增和酶切试验检测,表明SDS法所提DNA浓度较高,质量较好;CTAB法提取的DNA中氯仿等残留量少,安全、经济,具有较强的实用性;而碱煮法提取的DNA浓度较低,但提取过程简单,使用的药品少,快速,适合用于PCR快速检测所需DNA。

The genomic DNA was extracted from dry seed of waxy maize embryo as material while using the method of SDS, CTAB and NaOH buffer.After detected by UV spectrophotometer and agarose gel electrophoresis, the DNA extracted by the SDS method had high purity and integrity, and the DNA extraction of CTAB buffer had advantages of economy, safety with high practicality,which could completely meet the needs of RAPD-PCR and the digestion of the restriction endonuclease.But the extracted DNA using NaOH buffer were low concentra- tion,which suitable for the required DNA of the rapid detection of PCR with advantages of simple,rapid process, and less drugs.