不同产地龙眼叶中3种黄酮类物质含量测定

Determination of Quercetin,Luteolin and Kaempferol in the Leaf of Dimocarpus longan Lour.from Different Habitats

目的建立测定广西不同产地龙眼叶中槲皮素、木犀草素和山奈素含量的高效液相色谱法。方法色谱柱为Hypersil ODS C18柱(250 mm×4.6 mm,5μm),以甲醇-0.2%磷酸(47∶53)为流动相;流速1.0 mL.min-1;柱温:30℃;检测波长360 nm。结果槲皮素、木犀草素和山奈素分别在0.18～2.88,0.059～0.944和0.024～0.384μg之间呈良好的线性关系(r=0.999 9),平均加样回收率分别为100.68%,100.43%,100.30%,RSD分别为1.2%,2.2%,1.8%(n=9)。结论所建立的方法简便可靠,快速,重复性好,可用于龙眼叶的质量控制。

Objective To develop a method for content determination of quercetin, luteolin and kaempferol in the leaf of Dimocarpus longan Lout. from different habitats of Guangxi by HPLC. Methods The samples were separated on the Hypersil ODS C18（250 mm×4.6 mm,5 μm） colunm and eluted with methanol-0.2% phosphoric acid solution （47 ： 53） with the flow rate of 1.0 mL · min-1 and column temperature of 30 ℃. The detection wavelength was 360 ran. Results The method had good linear relationship within the range of 0.18-2.88 μg for quercetin ,0. 059-0.944 μg for luteolin and 0. 024 - 0. 384 μg （r=0. 999 9） for kaempferol. The average recovery rate was 100.68% （RSD= 1.2% ）, 100.43% （RSD = 2.2% ） and 100.30% （ RSD = 1.8% ） , respectively. Conclusion The method is simple, rapid and reliable, and can be used to deteixnine quercetin, luteolin and kaempferol in the leaf of Dimocarpus longan Lour.