摘要
目的建立测定广西不同产地龙眼叶中槲皮素、木犀草素和山奈素含量的高效液相色谱法。方法色谱柱为Hypersil ODS C18柱(250 mm×4.6 mm,5μm),以甲醇-0.2%磷酸(47∶53)为流动相;流速1.0 mL.min-1;柱温:30℃;检测波长360 nm。结果槲皮素、木犀草素和山奈素分别在0.18~2.88,0.059~0.944和0.024~0.384μg之间呈良好的线性关系(r=0.999 9),平均加样回收率分别为100.68%,100.43%,100.30%,RSD分别为1.2%,2.2%,1.8%(n=9)。结论所建立的方法简便可靠,快速,重复性好,可用于龙眼叶的质量控制。
Objective To develop a method for content determination of quercetin, luteolin and kaempferol in the leaf of Dimocarpus longan Lout. from different habitats of Guangxi by HPLC. Methods The samples were separated on the Hypersil ODS C18(250 mm×4.6 mm,5 μm) colunm and eluted with methanol-0.2% phosphoric acid solution (47 : 53) with the flow rate of 1.0 mL · min-1 and column temperature of 30 ℃. The detection wavelength was 360 ran. Results The method had good linear relationship within the range of 0.18-2.88 μg for quercetin ,0. 059-0.944 μg for luteolin and 0. 024 - 0. 384 μg (r=0. 999 9) for kaempferol. The average recovery rate was 100.68% (RSD= 1.2% ), 100.43% (RSD = 2.2% ) and 100.30% ( RSD = 1.8% ) , respectively. Conclusion The method is simple, rapid and reliable, and can be used to deteixnine quercetin, luteolin and kaempferol in the leaf of Dimocarpus longan Lour.
出处
《医药导报》
CAS
北大核心
2013年第8期1064-1066,共3页
Herald of Medicine
基金
广西自然科学基金项目(2010GXNSFB013071)
2009年度广西高校优秀人才资助计划
广西研究生创新计划资助项目(JGY2012032)
广西高等学校特色专业及课程一体化建设项目--制药工程(GXTSZY014)
关键词
龙眼叶
槲皮素
木犀草素
山奈素
含量测定
Dimocarpus longan Lour.
Quercetin
Luteolin
Kaempferol
Content determination