期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

流式细胞仪分离精子的应用现状 被引量：2

下载PDF
导出
摘要 流式细胞仪分离法具有准确性强、分离效率高、易于重复,技术比较先进可靠等优点,是目前广泛应用于畜牧业生产的精子分离方法。本文简要介绍了流式细胞仪分离精子的发展概况、原理方法、应用研究及生产应用等,并对其发展进行了展望。
作者 陈军 邓强 韩东 程相鲁 程黎明
机构地区 新疆维吾尔自治区畜牧总站
出处 《新疆畜牧业》 2013年第6期7-9,共3页
关键词 流式细胞仪 精子 性控
分类号 S814 [农业科学—畜牧学]
  • 相关文献

参考文献10

  • 1Pinkel D, Gledhill BL, Dilla MA, et al. High resolution DNA content measurements of mammalian sperm. Cytometry, 1983, (3) : 1-9.
  • 2Johnson la, pinkel d, medification of lascr-based flow cytometer for high resolution DNA analysis of mammalian spemlatozoa [J]. cytometry. 1986, (7) : 258-273.
  • 3Johnson la, flook jp, hawk hw. Sex preselection in rabbits: live births from x and y spermatozoa separated by DNA and cell sorting [J].boil reprod, 1989, 41 : 199-203.
  • 4Johnson LA, Flook JP, Hawk HW. Sex preselection in rabbits: live births from X and Y sperm separated by DNA and cell sorting. Bio Reprod, 1989, 41 : 199-203.
  • 5Johnson R C. Correction of semen variables and pregnancy rats for donor insemination: a 15-year retrospective . FertilSteril, 1994, 61. 355-359.
  • 6卢克焕.哺乳动物性别控制研究进展[J].中国兽医学报,2002,22(4):411-414. 被引量：19
  • 7Welch GR, Jhnson LA. Sex preselection: laboratory validation of the sperm sexing ratio of flow sorted X and Y sperm by sort reanalysis for DNA. Theriogenology, 1999, 52:1 342-1 352.
  • 8Johnson LA, Flook JP, Look MV. Flow analysis of X and Y chromosome-bearing sperm for DNA using an improved preparation method and staining with Hoechst 33342. Gamete Research, 1987b, 17: 203-212.
  • 9张明,卢克焕,G.E.Seidel,Jr.牛分离精子对体外受精的影响[J].广西农业生物科学,2002,21(4):223-228. 被引量：12
  • 10黄河,李鑫,扈长春,胡莉萍,罗应荣.应用X精子冷冻精液生产体内性控胚胎的试验研究进展[J].中国畜牧兽医,2005,32(7):27-30. 被引量：12

二级参考文献59

  • 1[1]Guyer M F. Accessory chromosomes in man[J]. Biol Bull Marine BiolLab. 1910.19:219.
  • 2[2]Lush J L. The possibiity of sex control by artificial insemination withcentrifuged spermatozoa[J]. J AgriRes, 1925, 30:893-913.
  • 3[3]Johnson L A. Flow cytometric determination of sperm sex ratio in semenpurportedly enriched for X or Y bearing sperm [J]. Theriogenology,1988.29:265.
  • 4[4]Pinkel D. Gledhill B L, Lake S, et al. High resolution DNA measurements of mammalian sperm[J]. Cytometry, 1982,3:1-9.
  • 5[5]Upreti G C, Riches P C, Johnson L A. Attempted sexing of bovine spermatozoa by fractionation on a percoll density gradient[J]. Gamete Res,1988,20:83-92.
  • 6[6]Avery O T. Macleod C M, McCarty M. Studies on the chemical nature ofthe substance inducing transformation of pneumococcal types. Inductionof transformation by a deoxyribonucleic acid fraction isolated from pneumococus Type Ⅲ[J]. J ExpMed,1944,79:137.
  • 7[7]Kamentsky L A. Melamed R R. Spectrophotometric cell sorter[J]. Science.1967, 156; 1364-1365.
  • 8[8]Gledhill B L.Lake S,Steinmetz L L, et al. Flow microfltuorometric analysis of sperm DNA content: effect of cell shape on the fluorescence distribution[J]. J Cellular Physiol.1976, 87:367-376.
  • 9[9]Moruzzi J F. Selecting a mammalian species for the separation of X- andY-chromosome-bearing spermatozoa[J]. J Reprod Steril, 1979,57: 319-323.
  • 10[10]Garner D L. Gledhill B L, Pinkel D, et al. Quantification of the X andY chromosome-bearing spermatozoa of domestic animals by flow cytometry[J]. BiolReprod,1983, 28: 312-321.

共引文献39

同被引文献42

  • 1郑春静,吴雄飞,刘东海,张顺.用流式细胞仪检测大黄鱼三倍体[J].细胞生物学杂志,2006,28(2):253-256. 被引量：11
  • 2孙玲.电泳法分离人类精子[J].国外医学（计划生育．生殖健康分册）,2006,25(4):234-235. 被引量：2
  • 3李乐,伍严安,黄源茂.胸腹水脱落细胞流式细胞法DNA倍体分析的临床诊断价值[J].福建医科大学学报,2007,41(2):159-162. 被引量：5
  • 4Fulwyler MJ. Flow cytometry and cell sorting [ J ]. Blood Cells, 1980, 6(2) :173-184.
  • 5Villas BH. Flow cytometry: an overview[ J]. Cell Vis, 1998,5 ( 1 ) :56-61.
  • 6Adan A, Alizada G, Kiraz Y, et al. Flow cytometry : basic principles and applications [ J ]. Crit Rev Biotechnol, 2016, 14 (1) :1-14. DOI:10. 3109/07388551. 2015. 1128876.
  • 7Swerdlow SH, Campo E, Harris NL, et al. WHO classification of haematopoiefic and lymphoid tissues [ M]. 4th edn. Lyon: International Agency for Research on Ccancer, 2008.
  • 8Sabattini E, Bacci F, Sagramoso C, et al. WHO classification of turnouts of haematopoietic and lymphoid tissues in 2008: an overview [ J ]. Pathologica, 201 O, 102 ( 3 ) : 83-87.
  • 9Chen J, Xue C, Zhao Y, et al. Microfluidic impedance flow cytometry enabling high-throughput single-cell electrical property characterization[J]. Int J Mol Sci, 2015, 16 (5) :9804-9830. DOL: 10. 3390/ijms16059804.
  • 10Doan H, Chinn GM, Jahan-Tigh RR. Flow Cytometry II: Mass and Imaging Cytometry[ J]. J Invest DermatoL, 2015, [35 (9) : e36. DOI: 10. 1038/jid. 2015. 263.

引证文献2

二级引证文献16

新疆畜牧业
2013年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部