β-catenin在肝癌中的表达和意义

Expression of β-catenin and its significance in liver carcinoma

目的β-catenin在胞内异常表达常见于多种原发性肿瘤,本研究拟观察β-catenin在原发性肝癌中的表达,探讨β-catenin在原发性肝癌发生发展中的作用。方法免疫组织化学Envsion法检测33例肝细胞癌及其癌旁组织,22例胆管细胞癌,20例转移性癌及20例正常肝组织中β-catenin蛋白的表达。结果β-catenin蛋白在肝细胞癌胞膜/胞质/胞核的阳性表达率分别是72%(24/33),76%(25/33)和39%(13/33);在胆管细胞癌胞膜/胞质/胞核的阳性表达率分别为86%(19/22),86%(19/22)和45%(10/22);在转移性癌胞膜/胞质/胞核的阳性表达率分别是80%(16/20),90%(18/20)和40%(8/20);在癌旁和正常肝组织中胞膜/胞质/胞核的表达率分别是58%(19/33),67%(22/33),0%(0/33)和60%(12/20),70%(14/20),0%(0/20)。β-catenin蛋白胞膜/胞质/胞核在肝细胞癌和胆管细胞癌中的表达与癌旁和正常肝组织胞膜/胞质/胞核的表达两两比较差异有统计学意义(P=0.000)。结论β-catenin的过表达提示其在原发性肝癌的发生发展过程中起着促癌的作用。

Objective Abnormal expression of β-catenin was often found in many kinds of primary tumors.The aim of this study was to examine the expression of β-catenin in hepatocellular carcinoma（HCC） and cholangiocellular carcinoma（CC）,and analyze their relationship with the genesis and development of primary liver carcinoma.Methods Immunohistochemical detection of β-catenin was performed in 33 HCC,22 CC,20 metastatic hepatic carcinoma（MHC）,33 adjacent non-tumor livers and 20 normal livers.Results The rate of expression was72%,86%,80%,58%,79%,60% for membranous β-catenin,and 76%,86%,90%,67%,70%for cytoplasmic β-catenin,and 39%,45%,40%,0% and 0% for nuclear β-catenin respectively in HCC,CC,MHC,adjacent non-tumor liver and normal liver.The positive rate of membranous/cytoplasmic/nuclearβ-catenin was significantly higher in HCC,CC and MHC than in adjacent non-tumor livers and normal livers（P=0.000）.Conclusion β-catenin was frequently high expressed in hepatocellular carcinoma and cholangiocellular carcinoma suggesting that overexpression of β-catenin may sustain the tumor progression.