新鲜菊苣提取和纯化菊粉的工艺

Technology of Extraction and Purification of Inulin from Fresh Chicory Root

研究比色法测定菊粉含量的方法,并采用正交设计优选了从新鲜菊苣根中热水浸提菊粉的提取工艺条件和脱蛋白以及脱色的最佳条件。实验结果表明,菊粉提取的最佳工艺条件为:料液比1∶1,浸泡时间40 min,加热浸提温度为100℃,加热浸提时间40 min,菊粉的提取率达14.74%;脱蛋白时菊液与活性炭的最佳比例为1∶1.5;提取液最佳脱色工艺条件为:脱色温度70℃,脱色时间50 min,活性碳用量为30 g/L,脱色率为74.36%。

The method of determination of insulin content using colorimeter wan studied, and the optimal condition of extracting inulin from fresh chicory roots with hot water and removing protein and colour was studied and selected by orthogonal design. The results showed the optimum process conditions of inulin extracting were as follow： solid-liquid ratio was 1 ： 1, immersion time was 40 min, heating extraction temperature was 100 ℃ and time was 40 min, the extraction rate of inulin from chicory root could achieved 14.74 % ; the optimum proportion of active carbon and inulin on removing protein was1 ： 1.5 ; the optimal removing colour condition were as follow： temperature was 70 ℃ ,time was 50 min, quantity of active carbon was 30 g/L, and decolourizing rate reached 74.36 %.